Icahn School Of Medicine At Mount Sinai
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ELISA
IHC
Antigen/Gene or Protein Targets: Glutamate Transporter EAAC1
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![Adapted from van Dinther et al. 2013. PLoS One. 8(4):e62295. PMID: 23638027 Figure. Sclerostin differentially interacts with LRP5/6.
(A) C2C12, C3H10T1/2, ATDC5 and KS483 were affinity labeled using [125I]Sclerostin, followed by chemical crosslinking. Cells were lysed and an immunoprecipitation was performed using LRP5 and LRP6 antibodies. Samples were separated on a SDS-PAGE gel and the radioactive signal was visualized using a phosphorimager screen. The unknown 50 kD band is marked with an asteriks on the short exposure of the KS483 input. (B) C2C12, C3H10T1/2, ATDC5 and KS483 cells were lysed and Western blot was performed to determine the expression of LRP5 and LRP6, GAPDH was used as a loading control. (C) Lysates were made of KS483 control cells (pLKO and NT-shRNA) and KS483 cells with stable LRP5 or LRP6 knockdown. Knockdown efficiency was determined by Western blotting. (D) KS483 control cells and KS483 cells with stable LRP5 or LRP6 knockdown were affinity labeled using [125I]Sclerostin, followed by chemical crosslinking. Cells were lysed and an immunoprecipitation was performed using LRP5 and LRP6 antibodies. LRP5 and LRP6 antibodies do not cross-react (Fig. S1a). Samples were separated on a SDS-PAGE gel and the radioactive signal was visualized using a phosphorimager screen. (E) High molecular weight bands corresponding to LRP5 and LRP6 were quantified using ImageQuant TL v2003.03 software (Amersham Biosciences), and normalized to the pLKO control.](https://res.cloudinary.com/ximbio/image/upload/c_thumb,fl_lossy,h_124,q_auto,r_max,w_124/39bff6ea-5319-4099-8c34-a38e977f82ef.jpg)
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ELISA
IHC
Antigen/Gene or Protein Targets: Glutamate Transporter EAAC1
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