Anti-APC [Ali 12-28]
Invented by Prof Walter Bodmer from University of Oxford
Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields
- Datasheet
- References (9)
- Inventor Info
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| Catalogue Number | 151073 |
| Applications | ELISA IHC IF IP WB |
| Antigen/Gene or Protein Targets | Adenomatous Polyposis Coli (APC) |
| Reactivity | Human |
| Relevance | The adenomatous polyposis coli (APC) tumour suppressor gene is mutated (often by deletion of the C-terminal portion of APC) in the inherited disease, familial adenomatous polyposis (FAP), and in over 80% of colorectal cancers. Ali 12-28 may be used for the detection of APC expression and APC mutations. |
| Host | Mouse |
| Immunogen | N-terminal APC-MBP fusion protein |
| Positive Control | Cancer cell lines: SW480, LOVO, DLD1, SW837 |
| Subclass | IgG1 |
| Molecular Weight (kDa) | 310 |
| Recommended Growing Conditions | DMEM + 5% FCS |
| Strain | Balb/c |
| Notes |
Ali 12-28 detects both normal and mutant-truncated forms of the APC. Recommended concentrations for western blot: 4 µg/ml Immunofluorescence: 20 µg/ml |
| Research Area | Cancer, Cardiovascular, Cell Cycle, Cell Type or Organelle Marker, Cell Signaling & Signal Transduction, Epigenetics & Nuclear Signalling, Neurobiology, Stem Cell Biology |
References: 9 entries
Hadjihannas et al. 2012. EMBO Rep. 13(4):347-54. PMID: 22322943.
Cell cycle control of Wnt/β-catenin signalling by conductin/axin2 through CDC20.
Europe PMC ID: 22322943
Valvezan et al. 2012. J Biol Chem. 287(6):3823-32. PMID: 22184111.
WB
Tanneberger et al. 2011. J Biol Chem. 286(22):19204-14. PMID: 21498506.
Structural and functional characterization of the Wnt inhibitor APC membrane recruitment 1 (Amer1).
Europe PMC ID: 21498506
Arnold et al. 2009. EMBO J. 28(5):500-12. PMID: 19131971.
The Axin1 scaffold protein promotes formation of a degradation complex for c-Myc.
Europe PMC ID: 19131971
Efstathiou et al. 1998. Proc Natl Acad Sci U S A. 95(6):3122-7. PMID: 9501226.
Intestinal trefoil factor controls the expression of the adenomatous polyposis coli-catenin and the E-cadherin-catenin complexes in human colon carcinoma cells.
Europe PMC ID: 9501226
Add a reference
References: 9 entries
Hadjihannas et al. 2012. EMBO Rep. 13(4):347-54. PMID: 22322943.
Cell cycle control of Wnt/β-catenin signalling by conductin/axin2 through CDC20.
Valvezan et al. 2012. J Biol Chem. 287(6):3823-32. PMID: 22184111.
WB
Tanneberger et al. 2011. J Biol Chem. 286(22):19204-14. PMID: 21498506.
Structural and functional characterization of the Wnt inhibitor APC membrane recruitment 1 (Amer1).
Arnold et al. 2009. EMBO J. 28(5):500-12. PMID: 19131971.
The Axin1 scaffold protein promotes formation of a degradation complex for c-Myc.
Efstathiou et al. 1998. Proc Natl Acad Sci U S A. 95(6):3122-7. PMID: 9501226.
Intestinal trefoil factor controls the expression of the adenomatous polyposis coli-catenin and the E-cadherin-catenin complexes in human colon carcinoma cells.
Add a reference
