Anti-Keratin14 [LL001]
Invented by Prof Irene Leigh from Queen Mary University of London
Invented at Queen Mary University of London
- Datasheet
- References (10)
- Inventor Info
Info
| Catalogue Number | 151121 |
| Applications | IHC IF IP WB |
| Antigen/Gene or Protein Targets | Keratin 14 |
| Reactivity | Human, Mouse and Pig |
| Relevance | Keratin 14, a type 1 intermediate filament protein, is one of the two keratins which distinguish stratifying epithelial cell types from simple epithelial cell types. This protein can be detected in intermediate filaments and stratified squamous epithelial cells; in the basal (myoepithelial) cells of glandular epithelia and all cells of keratinocyte lineage, including tumours. LL001 therefore characterises stratifying/keratinocyte cell types. |
| Host | Mouse |
| Immunogen | Peptide (last 16 carboxy-terminal amino acids of human keratin) coupled to thyroglobulin by the extra Cys added at the N terminus. |
| Subclass | IgG2a kappa |
| Molecular Weight (kDa) | 50 |
| Myeloma Used | Sp2/0-Ag14 |
| Recommended Growing Conditions | DMEM + 5% FCS |
| Strain | Balb/c |
| Notes | LL001 may be used on formalin fixed sections but is more effective on unfixed frozen sections. |
| Research Area | Cell Signaling & Signal Transduction |
References: 10 entries
Sun et al. 2011. Endocrinology. 152(8):3233-45. PMID: 21628386.
Decreased IGF type 1 receptor signaling in mammary epithelium during pregnancy leads to reduced proliferation, alveolar differentiation, and expression of insulin receptor substrate (IRS)-1 and IRS-2.
Europe PMC ID: 21628386
Karashima et al. 2002. J Cell Sci. 115(Pt 24):5027-37. PMID: 12432088.
Interaction of periplakin and envoplakin with intermediate filaments.
Europe PMC ID: 12432088
D'Alessandro et al. 2002. J Cell Sci. 115(Pt 22):4341-51. PMID: 12376565.
Keratin mutations of epidermolysis bullosa simplex alter the kinetics of stress response to osmotic shock.
Europe PMC ID: 12376565
Purkis et al. 1990. J Cell Sci. 97 ( Pt 1):39-50. PMID: 1701769.
Antibody markers of basal cells in complex epithelia.
Europe PMC ID: 1701769
Leigh et al. 1988. J Invest Dermatol. 90(5):639-42. PMID: 3283248.
Type VII collagen is a normal component of epidermal basement membrane, which shows altered expression in recessive dystrophic epidermolysis bullosa.
Europe PMC ID: 3283248
Add a reference
References: 10 entries
Sun et al. 2011. Endocrinology. 152(8):3233-45. PMID: 21628386.
Decreased IGF type 1 receptor signaling in mammary epithelium during pregnancy leads to reduced proliferation, alveolar differentiation, and expression of insulin receptor substrate (IRS)-1 and IRS-2.
Karashima et al. 2002. J Cell Sci. 115(Pt 24):5027-37. PMID: 12432088.
Interaction of periplakin and envoplakin with intermediate filaments.
D'Alessandro et al. 2002. J Cell Sci. 115(Pt 22):4341-51. PMID: 12376565.
Keratin mutations of epidermolysis bullosa simplex alter the kinetics of stress response to osmotic shock.
Purkis et al. 1990. J Cell Sci. 97 ( Pt 1):39-50. PMID: 1701769.
Antibody markers of basal cells in complex epithelia.
Leigh et al. 1988. J Invest Dermatol. 90(5):639-42. PMID: 3283248.
Type VII collagen is a normal component of epidermal basement membrane, which shows altered expression in recessive dystrophic epidermolysis bullosa.
Add a reference
