Anti-HPV16 E1/E4 [TVG 402]
Invented at University of Cambridge
- Datasheet
- References (4)
- Inventor Info
Info
| Catalogue Number | 151171 |
| Applications | ELISA IHC IP WB |
| Antigen/Gene or Protein Targets | Human Papilloma Virus 16 early proteins 1 and 4 (HPV16 E1/E4) |
| Reactivity | Human papilloma virus |
| Relevance | The human papilloma virus (HPV) family of DNA tumor viruses includes HPV-16 and HPV-18, which are associated with a large proportion of cervical cancer case. E1 and E2 are proteins involved in the regulation of viral DNA replication. TVG 402 and TVG 403 were the first antibodies against HPV16 E1 & E4 and should complement those already available to E7 and L1 for the screening of frozen sections of clinical biopsies. They will be of value in monitoring the progress of HPV infection from benign lesions to invasive cancer. |
| Host | Mouse |
| Immunogen | Antigen for hybridoma production was expressed as a b galactosidase fusion protein using the pEX expression system and was consequently cleaved to release the E1/E4 polypeptide. |
| Subclass | IgG1 |
| Molecular Weight (kDa) | 10 |
| Myeloma Used | NS0 |
| Recommended Growing Conditions | RPMI 1640 + 10% FCS, may need suppliment with SIGMA ITS medium. |
| Notes | The epitope recognised by this antibody survived brief fixation in 5% formaldehyde, but not prolonged fixation. |
| Research Area | Cancer, Virology |
References: 4 entries
Doorbar et al. 2000. J Virol. 74(21):10081-95. PMID: 11024137.
The E1E4 protein of human papillomavirus type 16 associates with a putative RNA helicase through sequences in its C terminus.
Europe PMC ID: 11024137
Doorbar et al. 1992. Virology. 187(1):353-9. PMID: 1371027.
Epitope-mapped monoclonal antibodies against the HPV16E1--E4 protein.
Europe PMC ID: 1371027
Add a reference
References: 4 entries
Doorbar et al. 2000. J Virol. 74(21):10081-95. PMID: 11024137.
The E1E4 protein of human papillomavirus type 16 associates with a putative RNA helicase through sequences in its C terminus.
Doorbar et al. 1992. Virology. 187(1):353-9. PMID: 1371027.
Epitope-mapped monoclonal antibodies against the HPV16E1--E4 protein.
Add a reference
