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Monoclonal Antibodies

Anti-CD45RO [UCH-L1]

Invented by Prof Peter Beverley from Imperial College
Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields
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Antigen cell-surface expression in CD45R0+ and CD45RBC+ HPB-ALL sub-clones.CD45R0+ (continuous line) or CD45RBC+ (dotted line) HPB-ALL cells were stained for CD45 as shown and analyzed by FACS. Adapted from Dornan et al J Biol Chem. 2002 Jan 18;277(3):1912-8. doi: 10.1074/jbc.M108386200. Epub 2001 Nov 2.
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Image thumbnail for Anti-CD45RO [UCH-L1]
Catalogue Number 151173
Applications FACS IHC IP WB
Antigen/Gene or Protein Targets Protein tyrosine phosphatase, receptor type, C (PTPRC, CD45RO)
Synonyms Protein Tyrosine Phosphatase; Receptor Type C; CD45 Antigen; CD45; L-CA; T200; Protein Tyrosine Phosphatase; Receptor Type; C Polypeptide; T200 Leukocyte Common Antigen; T200 Glycoprotein; EC 3.1.3.48; GP180; CD45R; B220; LCA; LY5
Reactivity Human
Relevance Highly specific monoclonal antibody which can detect CD45RO, differentiating between subpopulations of T lymphocytes.

Background and Research Application
CD45RO (UCHL1) is also known as protein tyrosine phosphatase receptor type C (PTPRC) or leukocyte common antigen. It is a transmembrane pan-leukocyte protein with tyrosine phosphatase activity and is involved in the regulation of signal transduction in haematopoiesis and the threshold of T cell antigen receptor (TCR) signalling. This is via dephosphorylation of protein tyrosine kinases (e.g. Lck and Fyn).
Anti-CD45RO subdivides the helper/inducer and suppressor/cytotoxic populations of T-cells, identifying sub populations of T cells which proliferate to soluble antigen and aid with PWM-stimulated immunoglobulin synthesis and B-cell differentiation.
CD45RO is the smallest isoform of the CD45 glycoprotein, produced via alternative splicing of exons 4-7, and is expressed on activated and memory T lymphocytes (around 50% of all T lymphocytes), most granulocytes and monocytes and most mature T cell tumours. CD45RO facilitates T cell activation, however, it is not present on B or NK cells.
CD45 regulates Src-family kinases, integrin-mediated signal transduction pathways and can suppress JAK kinases, acting as a negative regulator of cytokine receptor signalling.
CD45RO may respond via proliferation to recall antigens, provide help for secondary antibody responses and produce greater variety of cytokines.
This antibody reacts with an epitope unique for CD45R0. It may identify abnormalities of functional T-cell subsets in immune disorders, be used in tumour diagnosis by FACS or immunohistology and has been very useful in studies of human T cell function and ontogeny.
Host Mouse
Immunogen Cultured T cells from an IL-2-dependent T-cell line (CA1) prepared from human peripheral blood activated with influenza virus.
Immunogen UniProt ID P08575
Subclass IgG2a
Molecular Weight (kDa) 180
Myeloma Used P3/NS1/1-Ag4.1
Recommended Growing Conditions RPMI + 10% FCS.
Strain Balb/c
Notes Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Points of Interest
Anti-CD45RO can differentiate between memory and naïve cells, which can be used to evaluate an immune response. It binds to immunising cultured T cells and may be used to identify abnormalities in functional T-cell subsets within immune disorders, offering a diagnostic function.
Previous uses of anti-CD45RO include tumour diagnosis via FACS or immune-histology, studies of human T cell function and ontogeny and identification of T vs B leukaemia.
Anti-CD45RO recognises a specific isoform present on a subset of cells, which can be used in diagnosis and identification of cell subsets.
Immunohistochemical staining has been undertaken with anti-CD45RO to evaluate T-lymphocytic immune reactions (e.g. in tumour microenvironment), in different disease states or cancer types.

Concentration
1mg/ml as standard
Research Area Cancer, Cell Type or Organelle Marker, Epitope Tags, Immunology

References

There are 13 reference entries for this reagent.

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References: 13 entries

Elgueta et al. 2019. Front Immunol. 10:981. PMID: 31118938.

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Moura et al. 2017. Cell Mol Immunol. 14(9):758-769. PMID: 26996067.

View  

Wu et al. 2015. Oncotarget. 6(30):28633-45. PMID: 26416419.

View  

Zlobec et al. 2013. J Transl Med. 11:104. PMID: 23627766.

View  

Next-generation tissue microarray (ngTMA) increases the quality of biomarker studies: an example using CD3, CD8, and CD45RO in the tumor microenvironment of six different solid tumor types.

Europe PMC ID: 23627766

View  

Dornan et al. 2002. J Biol Chem. 277(3):1912-8. PMID: 11694532.

View  

Differential association of CD45 isoforms with CD4 and CD8 regulates the actions of specific pools of p56lck tyrosine kinase in T cell antigen receptor signal transduction.

Europe PMC ID: 11694532

View  

Ishii et al. 2001. Proc Natl Acad Sci U S A. 98(21):12138-43. PMID: 11593028.

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CD26-mediated signaling for T cell activation occurs in lipid rafts through its association with CD45RO.

Europe PMC ID: 11593028

View  

Ponsford et al. 2001. Clin Exp Immunol. 124(2):315-22. PMID: 11422210.

View  

Differential responses of CD45+ve T-cell subsets to MBP in multiple sclerosis.

Europe PMC ID: 11422210

View  

Smith et al. 1986. Immunology. 58(1):63-70. PMID: 2423439.

View  

Functional subsets of human helper-inducer cells defined by a new monoclonal antibody, UCHL1.

Europe PMC ID: 2423439

View  

Add a reference

References: 13 entries

Elgueta et al. 2019. Front Immunol. 10:981. PMID: 31118938.

View  

Moura et al. 2017. Cell Mol Immunol. 14(9):758-769. PMID: 26996067.

View  

Wu et al. 2015. Oncotarget. 6(30):28633-45. PMID: 26416419.

View  

Zlobec et al. 2013. J Transl Med. 11:104. PMID: 23627766.

View  

Next-generation tissue microarray (ngTMA) increases the quality of biomarker studies: an example using CD3, CD8, and CD45RO in the tumor microenvironment of six different solid tumor types.

View  

Dornan et al. 2002. J Biol Chem. 277(3):1912-8. PMID: 11694532.

View  

Differential association of CD45 isoforms with CD4 and CD8 regulates the actions of specific pools of p56lck tyrosine kinase in T cell antigen receptor signal transduction.

View  

Ishii et al. 2001. Proc Natl Acad Sci U S A. 98(21):12138-43. PMID: 11593028.

View  

CD26-mediated signaling for T cell activation occurs in lipid rafts through its association with CD45RO.

View  

Ponsford et al. 2001. Clin Exp Immunol. 124(2):315-22. PMID: 11422210.

View  

Differential responses of CD45+ve T-cell subsets to MBP in multiple sclerosis.

View  

Smith et al. 1986. Immunology. 58(1):63-70. PMID: 2423439.

View  

Functional subsets of human helper-inducer cells defined by a new monoclonal antibody, UCHL1.

View  

Add a reference

Inventor Information