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Monoclonal Antibodies

Anti-AP2a [AP2a 8G8/5]

Invented by Helen Hurst
Invented at Queen Mary University of London
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Adapted from Bennett et al. 2009. PLoS One. 4(9):e6931. PMID: 19742317 Figure. AP-2α interacts with HDAC1/2, and HDAC activity is required for targeted methylation in HNSCC cells. a) ChIP analysis was performed using AP-2α, SP1, HDAC1, and HDAC2 antibodies in SCC22B cells with and without AP-2α downregulation. ChIP eluate was then utilized for quantitative PCR using CDH1, RASGRF2, MLH1, RASSF1A, and PTEN promoter primers. b) Western blot analysis demonstrating increased acetylation following Trichostatin A (TSA) treatment. c) Bisulfite sequencing analysis was performed in SCC22B cells before and after TSA treatment. Solid circles represent methylated CpGs; whereas, open circles represent unmethylated CpG sites. d) Graphical representation of the average methylation percentage in the 3 target genes analyzed. * P-value = 0.0452; ** P-value = 3.77×10−6.
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Catalogue Number 151254
Applications ChIP IHC WB
Antigen/Gene or Protein Targets Activating Protein 2 (AP-2) alpha (Human)
Reactivity Human
Relevance AP-2 is a family of developmentally regulated transcription factors which also play a role in breast cancer and melanoma. AP-2ß may be important in cardiac and kidney development. The AP-2 transcription factors form the OB2 complex that has been shown to up-regulate c-erb-B2 transcription.
Host Mouse
Immunogen Bacterialy expressed AP-2a/AP-2g fusion protein.
Positive Control Extract from breast cancer cell line ZR75-1
Subclass IgG1
Molecular Weight (kDa) 50
Myeloma Used Sp2/0-Ag14
Recommended Growing Conditions DMEM + 5% FCS
Strain Balb/c
Notes AP2a 8G8/5 is specific for the alpha form of AP-2.

Antigen recovery using microwave or pressure cooking required for paraffin embedded tissue.
Research Area Cancer, Cardiovascular, Epigenetics & Nuclear Signalling

References

There are 5 reference entries for this reagent.

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References: 5 entries

Personal communication

Nordentoft et al. 2011. BMC Cancer. 11:135. PMID: 21489314.

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Increased expression of transcription factor TFAP2α correlates with chemosensitivity in advanced bladder cancer.

Europe PMC ID: 21489314

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Bennett et al. 2009. PLoS One. 4(9):e6931. PMID: 19742317.

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AP-2alpha induces epigenetic silencing of tumor suppressive genes and microsatellite instability in head and neck squamous cell carcinoma.

Europe PMC ID: 19742317

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References: 5 entries

Personal communication

Nordentoft et al. 2011. BMC Cancer. 11:135. PMID: 21489314.

View  

Increased expression of transcription factor TFAP2α correlates with chemosensitivity in advanced bladder cancer.

View  

Bennett et al. 2009. PLoS One. 4(9):e6931. PMID: 19742317.

View  

AP-2alpha induces epigenetic silencing of tumor suppressive genes and microsatellite instability in head and neck squamous cell carcinoma.

View  

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Inventor Information