Anti-Rad51B [Rad51B 1E11/6]
Invented by Dr Stephen West from Francis Crick Institute
Invented at Cancer Research UK London Research Institute: Clare Hall Laboratories
- Datasheet
- References (2)
- Inventor Info
Info
| Catalogue Number | 151281 |
| Applications | WB |
| Antigen/Gene or Protein Targets | Rad51B |
| Reactivity | Human and Hamster |
| Relevance | RAD51B is a Rad51 paralog. RAD51 is a eukaryotic homologue of E. coli RecA, a recombinase, and a component of the homologous recombination DNA repair pathway. RAD51 forms a nucleoprotein filament (through binding RAD52 and single stranded DNA that are exposed following double strand breaks) that initiates recombination. RAD51B is also a component for the homologous recombination pathway. |
| Host | Mouse |
| Immunogen | Raised against His-tagged human Rad51B, overexpressed in e.coli and purified on a talon affinity column under denaturing conditions, followed by gel purification using SDS-PAGE. |
| Subclass | IgG1 |
| Myeloma Used | Sp2/0-Ag14 |
| Recommended Growing Conditions | DMEM + 5% FCS |
| Strain | Balb/c |
| Notes | Detects as little as 10ng of purified protein. Recommended for detection of Rad51B of human and hamster origin by Western Blotting (starting dilution 1:200, dilution range 1:100-1:1000) and immunoprecipitation [1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)]. |
| Research Area | DNA Damage and Repair, Epigenetics & Nuclear Signalling |
References: 2 entries
French et al. 2002. J Biol Chem. 277(22):19322-30. PMID: 11912211.
Role of mammalian RAD51L2 (RAD51C) in recombination and genetic stability.
Europe PMC ID: 11912211
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References: 2 entries
French et al. 2002. J Biol Chem. 277(22):19322-30. PMID: 11912211.
Role of mammalian RAD51L2 (RAD51C) in recombination and genetic stability.
Add a reference
