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Polyclonal Antibodies

Anti-RuvC, Polyclonal [SWE6]

Invented by Dr Stephen West from Francis Crick Institute
Invented at Cancer Research UK London Research Institute: Clare Hall Laboratories
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Adapted from Eggleston et al. 1997. Cell; 89(4):607-17. PMID: 9160752. Figure. RuvC (0.5 μg) was mixed as indicated and cross-linked with glutaraldehyde (+) as described in Experimental Procedures. Proteins were denatured and separated on a 10% SDS–polyacrylamide gel. They were then transferred onto a membrane and probed with polyclonal antibody against RuvC (lanes e–h).
Adapted from Davies et al. 1998. Curr Biol; 8(12):725-7. PMID: 9637927. Figure. Coimmunoprecipitation of RuvABC. Combinations of the three Ruvproteins (A, B and C) were incubated without DNA (−), with duplex DNA (D) or with Holliday junction X0 DNA (X). After incubation, 50 μl BSA (50 mg/ml) was added and protein–DNA complexes were immunoprecipitated (IP) using 20 μg (a) anti-RuvA, (b) anti-RuvB or (c) anti-RuvC monoclonal antibodies coupled to protein-G–Sepharose beads using dimethyl pimelimidate. Complexes were analysed by SDS–PAGE followed by western blotting with a mixture of rabbit anti-RuvA, anti-RuvB and anti-RuvC polyclonal antibodies.
Adapted from Davies et al. 1998. Curr Biol; 8(12):725-7. PMID: 9637927. Figure. (a) The Ruv proteins were added in the sequence indicated to reaction mixtures containing synthetic Holliday junction DNA (X0). (b) Sensitivity of the western blotting assay. RuvA, RuvB and RuvC (25 ng each) were separated by SDS–PAGE and detected by western blotting.
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Catalogue Number 151331
Applications IP Fn WB
Antigen/Gene or Protein Targets RuvC
Reactivity E.coli
Relevance RuvC (an endonuclease) cleaves Holliday junctions.
Host Rabbit
Immunogen purified E. coli RuvC protein
Formulation Serum
Notes Suggested dilution for western blot: 1:1000
Research Area DNA Damage and Repair, Epigenetics & Nuclear Signalling

References

There are 4 reference entries for this reagent.

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References: 4 entries

Davies et al. 1998. Curr Biol. 8(12):725-7. PMID: 9637927.

WB IP

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Formation of RuvABC-Holliday junction complexes in vitro.

Europe PMC ID: 9637927

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Eggleston et al. 1997. Cell. 89(4):607-17. PMID: 9160752.

Fn WB

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In vitro reconstitution of the late steps of genetic recombination in E. coli.

Europe PMC ID: 9160752

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References: 4 entries

Davies et al. 1998. Curr Biol. 8(12):725-7. PMID: 9637927.

WB IP

View  

Formation of RuvABC-Holliday junction complexes in vitro.

View  

Eggleston et al. 1997. Cell. 89(4):607-17. PMID: 9160752.

Fn WB

View  

In vitro reconstitution of the late steps of genetic recombination in E. coli.

View  

Add a reference

Inventor Information