FLYRD18 Cell Line
Invented by Mary Collins from University College London
Invented at The Institute of Cancer Research
- Datasheet
- References (5)
- Inventor Info
Info
| Catalogue Number | 151446 |
| Parental Line | HT 1080 |
| Host | Human |
| Disease Keywords | Fibrosarcoma |
| Model | Packaging |
| Relevance | The FLYRD18 Packaging cell line enables production of high-titer, human complement-resistant recombinant retroviruses, with significantly reduced probability of replication-competent retrovirus generation. HT1080-based packaging cell line enabling production of recombinant retroviral vectors with Moloney murine leukemia virus cores and cat endogenous virus RD114 virus envelopes. The vectors demonstrate high resistance to the inhibitory effects of human serum/complement, increasing penetration of the vector, and making the system ideal for in vivo gene transfer. |
| Production Details | Human; for details of production of FLYRD18 cell line see Cosset et al. 1995. Journal of Virology. 69:7430-36. PMID: 7494248 |
| Research Area | Gene Expression, Genetic Studies Tools |
| Growth/Phenotype Keywords | Recombinant retroviral production |
| Recommended Growing Conditions | For recommended growth and recombinant retrovirus production conditions see Cosset F et al, Journal of Virology, 1995, v69 pp7430-7436 & Takeuchi Y et al, Journal of Virology, 1994, v68 pp8001-8007 |
| Cellosaurus ID | CVCL_8871 |
References: 5 entries
Production of retroviral vectors for gene therapy with the human packaging cell line FLYRD18.
Cosset et al. 1995. J Virol. 69(12):7430-6. PMID: 7494248.
High-titer packaging cells producing recombinant retroviruses resistant to human serum.
Europe PMC ID: 7494248
Takeuchi et al. 1994. J Virol. 68(12):8001-7. PMID: 7966590.
Type C retrovirus inactivation by human complement is determined by both the viral genome and the producer cell.
Europe PMC ID: 7966590
Add a reference
References: 5 entries
Production of retroviral vectors for gene therapy with the human packaging cell line FLYRD18.
Cosset et al. 1995. J Virol. 69(12):7430-6. PMID: 7494248.
High-titer packaging cells producing recombinant retroviruses resistant to human serum.
Takeuchi et al. 1994. J Virol. 68(12):8001-7. PMID: 7966590.
Type C retrovirus inactivation by human complement is determined by both the viral genome and the producer cell.
Add a reference
