1-7HB2 Cell Line
Invented by Prof Joyce Taylor-Papadimitriou at Cancer Research UK London Research Institute: Lincoln's Inn Fields
1-7 CE1 Cell Line
Invented by Prof Joyce Taylor-Papadimitriou from King's College London , Prof Joy Burchell from King's College London
Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields
- Datasheet
- References (6)
- Inventor Info
Info
| Catalogue Number | 151503 |
| Antigen/Gene or Protein Targets | ERBB2 |
| Parental Line | MSTV1-7 HER2 |
| Host | Human |
| Tissue | Breast |
| Disease Keywords | Breast cancer |
| Model | Transgenic |
| Relevance | The human mammary luminal epithelial cell line MTSV1-7 was transfected with an ERBB2 expression vector, enabling over-expression of the ERBB2 proto-oncogene. It allows in vitro study of ERBB2 proto-oncogene over-expression in human mammary epithelia adn of the effects of ERBB2 over-expression on epithelial morphology & adhesion. The over-expression of the ERBB2 receptor inhibits morphogenesis in vitro, and induces reduced expression of surface adhesion molecules as E-Cadherin and a2 integrin, suggesting a role for ERBB2 over-expression in tumour progression and metastasis. |
| Production Details | MTSV1-7 transfected with SV40 plasmid DNA; MTSV1-7 ce1 line transfected with plasmid constructs for pSV2-erbB2 and pSV2neo. |
| Research Area | Adhesion, Cancer, Cell Signaling & Signal Transduction, Cell Structure and Motility, Drug Discovery & Development |
| Recommended Growing Conditions | DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + Insulin (bovine) at 10ug/ml +Hycrocortisone at 5ug/ml. |
| Cellosaurus ID | CVCL_3033 |
References: 6 entries
D'souza et al. 1994. Proc Natl Acad Sci U S A. 91(15):7202-6. PMID: 7913748.
Overexpression of ERBB2 in human mammary epithelial cells signals inhibition of transcription of the E-cadherin gene.
Europe PMC ID: 7913748
D'Souza et al. 1993. Oncogene. 8(7):1797-806. PMID: 8099725.
Collagen-induced morphogenesis and expression of the alpha 2-integrin subunit is inhibited in c-erbB2-transfected human mammary epithelial cells.
Europe PMC ID: 8099725
Bartek et al. 1991. Proc Natl Acad Sci U S A. 88(9):3520-4. PMID: 1708884.
Efficient immortalization of luminal epithelial cells from human mammary gland by introduction of simian virus 40 large tumor antigen with a recombinant retrovirus.
Europe PMC ID: 1708884
Add a reference
References: 6 entries
D'souza et al. 1994. Proc Natl Acad Sci U S A. 91(15):7202-6. PMID: 7913748.
Overexpression of ERBB2 in human mammary epithelial cells signals inhibition of transcription of the E-cadherin gene.
D'Souza et al. 1993. Oncogene. 8(7):1797-806. PMID: 8099725.
Collagen-induced morphogenesis and expression of the alpha 2-integrin subunit is inhibited in c-erbB2-transfected human mammary epithelial cells.
Bartek et al. 1991. Proc Natl Acad Sci U S A. 88(9):3520-4. PMID: 1708884.
Efficient immortalization of luminal epithelial cells from human mammary gland by introduction of simian virus 40 large tumor antigen with a recombinant retrovirus.
Add a reference
