CDH5(PAC)CreERT2 Mouse
Invented by Prof Ralf H. Adams from Max Planck Society
Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields
- Datasheet
- References (18)
- Inventor Info
Info
| Catalogue Number | 151520 |
| Antigen/Gene or Protein Targets | Estrogen receptor (ERT2) under the vascular endothelial cadherin (Cdh5(PAC)) promoter. |
| Disease Keywords | Cancer; angiogenesis; |
| Relevance | Cdh5(PAC)-CreERT2 enable efficient inducible conditional recombinase expression in embryonic and adult endothelial cells (tissue-specific loxP knockout/knockin/transgene). The Cdh5(PAC)-CreERT2 mouse is an ideal tool in the study of gene function in angiogenesis, atherosclerosis and neovascularisation. |
| Production Details | A Cdh5(PAC)-CreERT2 transgene vector, containing a genomic Cdh5(PAC) promoter fragment fused to a CreERT2 cDNA, was injected into fertilised embryos (C57BL/6 or FVB/N). Founder lines were back-crossed to establish mice heterozygous for the Cdh5(PAC)-CreERT2 transgene. |
| Conditional Description | Conditional Cre-ERT2 expression under Cdh5(PAC) promoter in endothelial cells; inducible Cre activity by treatment with hormone (tamoxifen), inducing translocation of Cre-ERT2 to nucleus. |
| Mouse Genetic Background/Cross History | C57BL6 or FVB/N |
| Zygosity | Heterozygous |
| Research Area | Cancer, Cardiovascular, Developmental Biology, Genetic Studies Tools, Neurobiology |
| Notes |
The Cdh5(PAC)-CreERT2 mouse exhibits tissue-specific expression of an inducible Cre-ERT2 fusion protein, enabling tamoxifen-induced Cre recombinase activity in vascular endothelial cells. Administration of tamoxifen induces nuclear translocation of the Cre-ERT2 fusion protein, and subsequent Cre recombinase activity, allowing knockout/knockin/transgene studies of loxP-flanked genes in vascular endothelial cells. Non-induced Cdh5(PAC)-CreERT2 mice demonstrate no Cre recombinase activity, while tamoxifen-induced Cdh5(PAC)-CreERT2 mice demonstrate high penetrance in vascular endothelial cells (95%+). |
References: 18 entries
Bréart et al. 2011. J Exp Med. 208(6):1267-78. PMID: 21576386.
Lipid phosphate phosphatase 3 enables efficient thymic egress.
Europe PMC ID: 21576386
Luna-Zurita et al. 2010. J Clin Invest. 120(10):3493-507. PMID: 20890042.
Pitulescu et al. 2010. Nat Protoc. 5(9):1518-34. PMID: 20725067.
Robson et al. 2010. Dev Dyn. 239(9):2435-42. PMID: 20652948.
Integration of a Notch-dependent mesenchymal gene program and Bmp2-driven cell invasiveness regulates murine cardiac valve formation.
Europe PMC ID: 20890042
The TGFβ type II receptor plays a critical role in the endothelial cells during cardiac development.
Europe PMC ID: 20652948
Inducible gene targeting in the neonatal vasculature and analysis of retinal angiogenesis in mice.
Europe PMC ID: 20725067
Wang et al. 2010. Nature. 465(7297):483-6. PMID: 20445537.
Ephrin-B2 controls VEGF-induced angiogenesis and lymphangiogenesis.
Europe PMC ID: 20445537
Mahmoud et al. 2010. Circ Res. 106(8):1425-33. PMID: 20224041.
Pathogenesis of arteriovenous malformations in the absence of endoglin.
Europe PMC ID: 20224041
Bazigou et al. 2009. Dev Cell. 17(2):175-86. PMID: 19686679.
Integrin-alpha9 is required for fibronectin matrix assembly during lymphatic valve morphogenesis.
Europe PMC ID: 19686679
Benedito et al. 2009. Cell. 137(6):1124-35. PMID: 19524514.
The notch ligands Dll4 and Jagged1 have opposing effects on angiogenesis.
Europe PMC ID: 19524514
Sörensen et al. 2009. Blood. 113(22):5680-8. PMID: 19144989.
DLL1-mediated Notch activation regulates endothelial identity in mouse fetal arteries.
Europe PMC ID: 19144989
Add a reference
References: 18 entries
Bréart et al. 2011. J Exp Med. 208(6):1267-78. PMID: 21576386.
Lipid phosphate phosphatase 3 enables efficient thymic egress.
Luna-Zurita et al. 2010. J Clin Invest. 120(10):3493-507. PMID: 20890042.
Pitulescu et al. 2010. Nat Protoc. 5(9):1518-34. PMID: 20725067.
Robson et al. 2010. Dev Dyn. 239(9):2435-42. PMID: 20652948.
Integration of a Notch-dependent mesenchymal gene program and Bmp2-driven cell invasiveness regulates murine cardiac valve formation.
The TGFβ type II receptor plays a critical role in the endothelial cells during cardiac development.
Inducible gene targeting in the neonatal vasculature and analysis of retinal angiogenesis in mice.
Wang et al. 2010. Nature. 465(7297):483-6. PMID: 20445537.
Ephrin-B2 controls VEGF-induced angiogenesis and lymphangiogenesis.
Mahmoud et al. 2010. Circ Res. 106(8):1425-33. PMID: 20224041.
Pathogenesis of arteriovenous malformations in the absence of endoglin.
Bazigou et al. 2009. Dev Cell. 17(2):175-86. PMID: 19686679.
Integrin-alpha9 is required for fibronectin matrix assembly during lymphatic valve morphogenesis.
Benedito et al. 2009. Cell. 137(6):1124-35. PMID: 19524514.
The notch ligands Dll4 and Jagged1 have opposing effects on angiogenesis.
Sörensen et al. 2009. Blood. 113(22):5680-8. PMID: 19144989.
DLL1-mediated Notch activation regulates endothelial identity in mouse fetal arteries.
Add a reference
