MEF UNG KO Cell Line
Invented at Cancer Research UK London Research Institute: Clare Hall Laboratories
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- References (2)
- Inventor Info
Info
| Catalogue Number | 151559 |
| Antigen/Gene or Protein Targets | Uracil-DNA Glycosylase (UNG) |
| Host | Mouse |
| Tissue | Embryo |
| Disease Keywords | Cancer, B cell lymphoma |
| Model | Knock-Out |
| Relevance | The MEF UNG KO Cell Line is a tool for in vivo study of the function of UNG and the effects of DNA mismatch during DNA synthesis. UNG encodes one of several uracil-DNA glycosylases. One important function of uracil-DNA glycosylases is to prevent mutagenesis by eliminating uracil from DNA molecules by cleaving the N-glycosylic bond and initiating the base-excision repair (BER) pathway. |
| Production Details | Primary MEFs were prepared from embryos, and permanent cell lines established from transformed clones arising spontaneously after repeated passage in culture, by standard procedures |
| Research Area | DNA Damage and Repair |
| Recommended Growing Conditions | DMEM:F12 (1:1) + 10% FCS. |
References: 2 entries
Nilsen et al. 2003. Oncogene. 22(35):5381-6. PMID: 12934097.
Gene-targeted mice lacking the Ung uracil-DNA glycosylase develop B-cell lymphomas.
Europe PMC ID: 12934097
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References: 2 entries
Nilsen et al. 2003. Oncogene. 22(35):5381-6. PMID: 12934097.
Gene-targeted mice lacking the Ung uracil-DNA glycosylase develop B-cell lymphomas.
Add a reference
