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Monoclonal Antibodies

Anti-SIGLEC5 [1A5]

Invented by Prof Paul Crocker from University of Dundee
Invented at University of Dundee
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Adapted from Nguyen et al. 2006 PNAS; 103(20): 7765–7770. PMID: 16682635 Figure. Jurkat cells were mock-transfected with no DNA or transfected with 2 μg of pSig5 per 2 × 106 cells using the Nucleofector device. After 24 h, Siglec-5 expression was analyzed by flow cytometry with anti-Siglec-5 and goat anti-mouse IgG Alexa Fluor 488, by using a nonspecific mouse IgG as background
Adapted from Cornish et al. 1998. Blood; 92 (6): 2123–2132. PMID: 9731071 Figure. Western blot analyses of neutrophil lysates with anti–siglec-5 antiserum. Samples, either reduced (R) or nonreduced (NR), were separated on a 10% polyacrylamide gel and transferred to nitrocellulose. The blot was incubated with anti–siglec-5 antiserum at 1:2,000 dilution or preimmune serum at 1:1,000 dilution, washed, and incubated with 125I-antirabbit IgG. No signal was observed with preimmune serum (not shown) but a predominant band at approximately 70 kD and approximately 140 kD can be seen under reducing and nonreducing conditions, respectively.
Adapted from Connolly et al. 2002. Br J Haemato; 119(1):221-38. PMID: 12358929 Figure. Tissue expression pattern of hSiglec‐5 using monoclonal antibody 1A5. Single labelling of peripheral blood and frozen sections was performed by the APAAP method and using the HRP EnVision kit respectively. Double fluorescent labelling was carried out using a two stage indirect method with hSiglec‐5 staining in green.
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Catalogue Number 151589
Applications ELISA FACS IHC IF IP Fn WB
Antigen/Gene or Protein Targets Siglec5
Synonyms Sialic Acid Binding Ig Like Lectin 5; Obesity-Binding Protein 2; OB-Binding Protein 2; CD33 Antigen-Like 2; SIGLEC-5; CD33L2; OBBP2; Sialic Acid-Binding Immunoglobulin-Like Lectin 5; CD170 Antigen;
Reactivity Human
Relevance The Siglecs are a family of membrane bound lectins (of the immunoglobulin superfamily) that bind sialic acid and mediate cell-cell interactions. Siglec5 (CD170) is a member of the recently-described human CD33-related siglec subgroup of sialic acid binding Ig-like lectins and is expressed on myeloid cells of the hemopoietic system. Similar to other CD33- related siglecs, Siglec-5 contains two tyrosine based motifs in its cytoplasmic tails implicated in signalling functions. The antibody cross reacts with Siglec14.
Host Mouse
Immunogen Siglec-5-Fc protein, containing entire extracellular region of siglec-5 fused with the Fc region of human IgG1
Positive Control Siglec5 transfected CHO cells. Apart from Siglec14 (Angata T et al. 2006 FASEB J. 20:1964-73. PMID: 17012248), non-cross-reactive with panel of other Siglec proteins, either expressed on CHO cells or as recombinant proteins.
Subclass IgG1
Myeloma Used Sp2/0-Ag14
Recommended Growing Conditions DMEM + 10% FetalClone I + 1% penicillin/streptomycin
Notes Recommended dilution for FACS: Cells (1x10^6) should be incubated with 1ug of antibody/100 ul in 1% BSA in PBS for 30–60 min on ice (Nguyen et al., 2006).
Research Area Adhesion, Cell Type or Organelle Marker, Immunology

References

There are 10 reference entries for this reagent.

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References: 10 entries

Angata et al. 2006. FASEB J. 20(12):1964-73. PMID: 17012248.

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Discovery of Siglec-14, a novel sialic acid receptor undergoing concerted evolution with Siglec-5 in primates.

Europe PMC ID: 17012248

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Nguyen et al. 2006. Proc Natl Acad Sci U S A. 103(20):7765-70. PMID: 16682635.

FACS

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Loss of Siglec expression on T lymphocytes during human evolution.

Europe PMC ID: 16682635

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Avril et al. 2005. J Biol Chem. 280(20):19843-51. PMID: 15769739.

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Siglec-5 (CD170) can mediate inhibitory signaling in the absence of immunoreceptor tyrosine-based inhibitory motif phosphorylation.

Europe PMC ID: 15769739

View  

Connolly et al. 2002. Br J Haematol. 119(1):221-38. PMID: 12358929

IHC

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Human Siglec-5: tissue distribution, novel isoforms and domain specificities for sialic acid-dependent ligand interactions.

Europe PMC ID: 12358929

View  

Cornish et al. 1998. Blood. 92(6):2123-32. PMID: 9731071.

View  

Characterization of siglec-5, a novel glycoprotein expressed on myeloid cells related to CD33.

Europe PMC ID: 9731071

View  

Add a reference

References: 10 entries

Angata et al. 2006. FASEB J. 20(12):1964-73. PMID: 17012248.

View  

Discovery of Siglec-14, a novel sialic acid receptor undergoing concerted evolution with Siglec-5 in primates.

View  

Nguyen et al. 2006. Proc Natl Acad Sci U S A. 103(20):7765-70. PMID: 16682635.

FACS

View  

Loss of Siglec expression on T lymphocytes during human evolution.

View  

Avril et al. 2005. J Biol Chem. 280(20):19843-51. PMID: 15769739.

View  

Siglec-5 (CD170) can mediate inhibitory signaling in the absence of immunoreceptor tyrosine-based inhibitory motif phosphorylation.

View  

Connolly et al. 2002. Br J Haematol. 119(1):221-38. PMID: 12358929

IHC

View  

Human Siglec-5: tissue distribution, novel isoforms and domain specificities for sialic acid-dependent ligand interactions.

View  

Cornish et al. 1998. Blood. 92(6):2123-32. PMID: 9731071.

View  

Characterization of siglec-5, a novel glycoprotein expressed on myeloid cells related to CD33.

View  

Add a reference

Inventor Information