| Catalogue Number | 151629 |
| Antigen/Gene or Protein Targets | Raf1 Y340F Y341F inactive mutant |
| Host | Mouse |
| Tissue | Embryo |
| Disease Keywords | Cancer |
| Model | Knock-In |
| Relevance | The MEF Raf1 FF/FF KI Cell Line is a MEF line obtained from the knock-in mouse carrying inactive Raf-1FF/FF mutant. |
| Production Details | Embryos were dissected and cut, washed several times with cold PBS and then incubated at 4°C for 2 hours in 0.25% trypsin. Cells were incubated as per the growth conditions. Primary cells were immortalised and resulting cells grown out by continuous culture. |
| Research Area | Cancer, Cell Signaling & Signal Transduction, Drug Discovery & Development, Metabolism |
| Recommended Growing Conditions | DMEM, 10% FCS, 100U/mol penicillin/streptomycin |
| Notes | See the Raf KI FF knockout mouse that these cells were derived from in related products. |
| Cellosaurus ID | CVCL_6D90 |
Invented at University Of Leicester
Hüser et al. 2001. EMBO J. 20(8):1940-51. PMID: 11296227.
MEK kinase activity is not necessary for Raf-1 function.
Europe PMC ID: 11296227
Hüser et al. 2001. EMBO J. 20(8):1940-51. PMID: 11296227.
MEK kinase activity is not necessary for Raf-1 function.
