| Catalogue Number | 151654 |
| Antigen/Gene or Protein Targets | Fibroblast growth factor receptor 2 isoform IIIb (FGFR2IIIb) |
| Disease Keywords | Cancer; gastric cancer; |
| Relevance | Conditional knockout of FGFR2 IIIb isoform; in vivo study of FGFR2 IIIb isoform knockout |
| Production Details | An Fgfr2 targeting vector, containing a loxP flanked IIIb exon and a loxP flanked resistance cassette, was transfected into 129Ola ES cells.Properly targeted ES cells containing a homologous recombination event were selected, and transfected with a cre expression vector to excise the resistance cassette. Targeted ES cells were injected into C57BL6 blastocysts. Chimeric offspring were mated to C57BL6 mice to generate heterozygotes. Heterozygotes were bred to homozygosity to generate Fgfr2bflox/flox mice. |
| Conditional | Yes |
| Conditional Description | LoxP flanked IIIb exon of FGFR2 gene enabling tissue-specific cre-mediated knockout of IIIb isoform. |
| Strain | C57BL/6 |
| Mouse Genetic Background/Cross History | 129/C57BL6 |
| Zygosity | Homozygous |
| Research Area | Cancer, Cell Signaling & Signal Transduction, Genetic Studies Tools, Immunology |
Grose et al. 2007. EMBO J. 26(5):1268-78. PMID: 17304214.
The role of fibroblast growth factor receptor 2b in skin homeostasis and cancer development.
Europe PMC ID: 17304214
De Moerlooze et al. 2000. Development. 127(3):483-92. PMID: 10631169.
An important role for the IIIb isoform of fibroblast growth factor receptor 2 (FGFR2) in mesenchymal-epithelial signalling during mouse organogenesis.
Europe PMC ID: 10631169
Grose et al. 2007. EMBO J. 26(5):1268-78. PMID: 17304214.
The role of fibroblast growth factor receptor 2b in skin homeostasis and cancer development.
De Moerlooze et al. 2000. Development. 127(3):483-92. PMID: 10631169.
An important role for the IIIb isoform of fibroblast growth factor receptor 2 (FGFR2) in mesenchymal-epithelial signalling during mouse organogenesis.
