MEF PKCe KO KI Cell Line
Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields
- Datasheet
- References (5)
- Inventor Info
Info
| Catalogue Number | 151663 |
| Antigen/Gene or Protein Targets | PKC epsilon |
| Host | Mouse |
| Tissue | Embryo |
| Disease Keywords | Cancer, heart disease |
| Model | Transgenic |
| Relevance | MEF PKCe KO KI Cell Line is a clonal MEF line derived from PKCepsilon knock-out embryos subsequently transfected to re-express PKCe through stable transfection with pcDNA3 CMV/IE hygo+ PKCe. Matched isogenic cell line which is null for PKCe is also available. Useful for studying the role of PKCe in various biological processes |
| Production Details | MEFs were derived from PKCe knockout embryos. Polyclonal PKCeKO cells were transfected with pcDNA3 CMV/IE hygro+ PKCe vector using calcium phosphate. Clonal stable cell lines were selected using limiting dilution. The vector was contructed by replacing the promoter region in pCMV hygro+ with a 2.1kb CMV/IE promoter derived from p63d. Mouse cDNA encoding full-length PKCe (3.3kb fragment) was ligated to the modified pcDNA3 CMV/IE hygro+ vector. |
| Research Area | Adhesion, Cell Signaling & Signal Transduction, Cell Structure and Motility |
| Growth/Phenotype Keywords | Normal phenotype |
| Recommended Growing Conditions | Cells were selected, and are routinely cultured in DMEM + 10% FCS and 100ug/ml hygromycin at 30degC in a 10% CO2 atmosphere |
| Cellosaurus ID | CVCL_AS82 |
References: 5 entries
Cited by 24 subsequent publications including:
Ivaska, J. et al. 2002. EMBO J. 21:3608-19.PMID: 12110574
Kermorgant, S. et al. 2004. EMBO J. 23: 3721:34. PMID 15385963
Pardo et al. 2006. EMBO J. 25(13):3078-88. PMID: 16810323.
FGF-2 protects small cell lung cancer cells from apoptosis through a complex involving PKCepsilon, B-Raf and S6K2.
Europe PMC ID: 16810323
Add a reference
References: 5 entries
Cited by 24 subsequent publications including:
Ivaska, J. et al. 2002. EMBO J. 21:3608-19.PMID: 12110574
Kermorgant, S. et al. 2004. EMBO J. 23: 3721:34. PMID 15385963
Pardo et al. 2006. EMBO J. 25(13):3078-88. PMID: 16810323.
FGF-2 protects small cell lung cancer cells from apoptosis through a complex involving PKCepsilon, B-Raf and S6K2.
Add a reference
