hTERT-RPE1 mRuby-APC15 Cell Line
Invented by Dr Jonathon Pines from Gurdon Institute
Invented at University of Cambridge
- Datasheet
- References (2)
- Inventor Info
Info
| Catalogue Number | 151710 |
| Antigen/Gene or Protein Targets | APC15 |
| Parental Line | hTERT-RPE1 |
| Host | Human |
| Tissue | Eye |
| Model | Transgenic |
| Relevance | The hTERT-RPE1 mRuby-APC15 Cell Line is a tet-inducible APC15 reporter cell line. APC15 is a component of the human cell cycle Anaphase promoting complex/cyclosome (APC/C). It is required for progression from metaphase during cell cycle. The uncharacterised open reading frame C11orf51 has been identified in a systematic proteomic analysis of APC/C purified from HeLa cell extracts. Human C11orf51 is conserved in vertebrates and invertebrates and has homology to Schizosaccharomyces pombe APC15 (PM gene ID: 2540655), and Schizosaccharomyces cerevisiae Mnd2 (PM gene ID: 854843). |
| Production Details | APC15 was amplified by PCR from a full length cDNA clone (ORFeome clone 100066673). Tetracycline inducible cell lines expressing APC15 IRES2 mRuby were generated using the FLIP in system and a modified pCDNA5/FRT/TO vector. The parental tetracycline inducible RPE cell line was created by random integration of an FRT site and a Tet repressor gene into hTERT RPE1 cells followed by selection of a clone that showed high levels of gene expression repression in the absence of tetracycline. |
| Conditional | Yes |
| Conditional Description | Tetracycline inducible |
| Research Area | Cell Cycle |
| Recommended Growing Conditions | F12/DMEM (1:1) media supplemented with 10% FCS, sodium bicarbonate, glutamate and penicillin/streptavidin. |
| Cellosaurus ID | CVCL_5F68 |
References: 2 entries
Mansfeld et al. 2011. Nat Cell Biol. 13(10):1234-43. PMID: 21926987.
APC15 drives the turnover of MCC-CDC20 to make the spindle assembly checkpoint responsive to kinetochore attachment.
Europe PMC ID: 21926987
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References: 2 entries
Mansfeld et al. 2011. Nat Cell Biol. 13(10):1234-43. PMID: 21926987.
APC15 drives the turnover of MCC-CDC20 to make the spindle assembly checkpoint responsive to kinetochore attachment.
Add a reference
