C75 Colorectal adenocarcinoma Cell Line
Invented by Prof Walter Bodmer from University of Oxford
Invented at University of Oxford
- Datasheet
- References (2)
- Inventor Info
Info
| Catalogue Number | 151758 |
| Host | Human |
| Tissue | Colon |
| Disease Keywords | Adenocarcnoma of the sigmoid colon |
| Model | Tumour line |
| Relevance | The C75 cell line was established from a 56-year old male patient with a moderately well differentiated adenocarcnoma of the sigmoid colon classified as Dukes' stage D |
| Research Area | Cancer, Drug Discovery & Development |
| Recommended Growing Conditions | Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37°C. Culture medium: Iscove's Modified Dulbecco's Medium, + 10% Foetal Bovine Serum (FBS) + 2 mM Glutamine |
| Cellosaurus ID | CVCL_5248 |
References: 2 entries
Browning et al. 1993. Proc Natl Acad Sci U S A. 90(7):2842-5. PMID: 8464898.
Tissue typing the HLA-A locus from genomic DNA by sequence-specific PCR: comparison of HLA genotype and surface expression on colorectal tumor cell lines.
Europe PMC ID: 8464898
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References: 2 entries
Browning et al. 1993. Proc Natl Acad Sci U S A. 90(7):2842-5. PMID: 8464898.
Tissue typing the HLA-A locus from genomic DNA by sequence-specific PCR: comparison of HLA genotype and surface expression on colorectal tumor cell lines.
Add a reference
