Anti-Desmoglein [33-3D]
Invented at University of Southampton
- Datasheet
- References (12)
- Inventor Info
Info
| Catalogue Number | 151794 |
| Applications | EM IHC IF WB |
| Antigen/Gene or Protein Targets | Desmoglein 1 and 2 |
| Reactivity | Bovine and Human |
| Relevance | Desmoglein 1 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Desmosomes are intercellular adhesive junctions that occur in almost all epithelia and should therefore be useful as epithelial markers in tumour diagnosis. 33-3D is directed against desmoglein in mammalian tissues, tumours and cultured cells. |
| Host | Mouse |
| Immunogen | Bovine desmoglein (desmosomal glycoprotein 1) from nasal epidermis |
| Positive Control | 33-3D was validated on MDCK cells which are fixed using methanol (Vilela et al 1995). |
| Subclass | IgM |
| Myeloma Used | P3/NS1/1-Ag4.1 |
| Recommended Growing Conditions | DMEM + 2mM glutamine + 20% FBS |
| Strain | Balb/c |
| Research Area | Adhesion, Cancer, Cell Signaling & Signal Transduction |
References: 12 entries
Krunic et al. 1998. Br J Cancer. 77(8):1275-9. PMID: 9579833.
IHC
Immunohistochemical staining for desmogleins 1 and 2 in keratinocytic neoplasms with squamous phenotype: actinic keratosis, keratoacanthoma and squamous cell carcinoma of the skin.
Europe PMC ID: 9579833
Krunic et al. 1996. Acta Derm Venereol. 76(5):394-8. PMID: 8891017.
IHC
Differential expression of desmosomal glycoproteins in keratoacanthoma and squamous cell carcinoma of the skin: an immunohistochemical aid to diagnosis.
Europe PMC ID: 8891017
Schäfer et al. 1996. Differentiation. 60(2):99-108. PMID: 8641550.
IHC
Immunological identification and characterization of the desmosomal cadherin Dsg2 in coupled and uncoupled epithelial cells and in human tissues.
Europe PMC ID: 8641550
Vilela et al. 1995. J Cell Sci. 108 ( Pt 4):1743-50. PMID: 7615689.
EM
A simple epithelial cell line (MDCK) shows heterogeneity of desmoglein isoforms, one resembling pemphigus vulgaris antigen.
Europe PMC ID: 7615689
Collins et al. 1990. Br J Cancer. 62(5):796-805. PMID: 2123114.
WB
A study of desmosomes in colorectal carcinoma.
Europe PMC ID: 2123114
Vilela et al. 1987. J Pathol. 153(4):365-75. PMID: 3323436.
IHC
Monoclonal antibody to desmosomal glycoprotein 1--a new epithelial marker for diagnostic pathology.
Europe PMC ID: 3323436
Add a reference
References: 12 entries
Krunic et al. 1998. Br J Cancer. 77(8):1275-9. PMID: 9579833.
IHC
Immunohistochemical staining for desmogleins 1 and 2 in keratinocytic neoplasms with squamous phenotype: actinic keratosis, keratoacanthoma and squamous cell carcinoma of the skin.
Krunic et al. 1996. Acta Derm Venereol. 76(5):394-8. PMID: 8891017.
IHC
Differential expression of desmosomal glycoproteins in keratoacanthoma and squamous cell carcinoma of the skin: an immunohistochemical aid to diagnosis.
Schäfer et al. 1996. Differentiation. 60(2):99-108. PMID: 8641550.
IHC
Immunological identification and characterization of the desmosomal cadherin Dsg2 in coupled and uncoupled epithelial cells and in human tissues.
Vilela et al. 1995. J Cell Sci. 108 ( Pt 4):1743-50. PMID: 7615689.
EM
A simple epithelial cell line (MDCK) shows heterogeneity of desmoglein isoforms, one resembling pemphigus vulgaris antigen.
Collins et al. 1990. Br J Cancer. 62(5):796-805. PMID: 2123114.
WB
A study of desmosomes in colorectal carcinoma.
Vilela et al. 1987. J Pathol. 153(4):365-75. PMID: 3323436.
IHC
Monoclonal antibody to desmosomal glycoprotein 1--a new epithelial marker for diagnostic pathology.
Add a reference
