Anti-PITPb [1C1]
Invented at University College London
- Datasheet
- References (6)
- Inventor Info
Info
| Catalogue Number | 151820 |
| Applications | ELISA IF WB |
| Antigen/Gene or Protein Targets | Rat PITPb |
| Reactivity | Human, Mouse, Rat and Dog |
| Relevance | Mammalian PITPß (phosphatidylinositol transfer protein ß) is a 272-amino-acid polypeptide capable of transferring PtdIns, PtdCho and SM (sphingomyelin) between membrane bilayers. Ablation of PITPß is embryonically lethal. This antibody is specific for both splice variants of PITPß but does not recognise PITPa despite the 95% similarity between the two. |
| Host | Mouse |
| Immunogen | Whole Rat PITPb protein |
| Subclass | IgG |
| Recommended Growing Conditions | RPMI 1640 + 10% FCS + 2mM L-glutamine + penicillin/streptomycin |
| Notes |
Antibody specific for both splice variants of PITPb but does not recognise PITPa. Production yield is high Does work in IF but not as good as DaRT/U/4a7 |
| Research Area | Cell Signaling & Signal Transduction |
References: 6 entries
Carvou et al. 2010. J Cell Sci. 123(Pt 8):1262-73. PMID: 20332109.
Phosphatidylinositol- and phosphatidylcholine-transfer activity of PITPbeta is essential for COPI-mediated retrograde transport from the Golgi to the endoplasmic reticulum.
Europe PMC ID: 20332109
Shadan et al. 2008. Traffic. 9(10):1743-56. PMID: 18636990.
Dynamics of lipid transfer by phosphatidylinositol transfer proteins in cells.
Europe PMC ID: 18636990
Morgan et al. 2006. Biochem J. 398(3):411-21. PMID: 16780419.
Differential expression of a C-terminal splice variant of phosphatidylinositol transfer protein beta lacking the constitutive-phosphorylated Ser262 that localizes to the Golgi compartment.
Europe PMC ID: 16780419
Add a reference
References: 6 entries
Carvou et al. 2010. J Cell Sci. 123(Pt 8):1262-73. PMID: 20332109.
Phosphatidylinositol- and phosphatidylcholine-transfer activity of PITPbeta is essential for COPI-mediated retrograde transport from the Golgi to the endoplasmic reticulum.
Shadan et al. 2008. Traffic. 9(10):1743-56. PMID: 18636990.
Dynamics of lipid transfer by phosphatidylinositol transfer proteins in cells.
Morgan et al. 2006. Biochem J. 398(3):411-21. PMID: 16780419.
Differential expression of a C-terminal splice variant of phosphatidylinositol transfer protein beta lacking the constitutive-phosphorylated Ser262 that localizes to the Golgi compartment.
Add a reference
