Cancer Research Technology
Log in Register
Menu
Search
Polyclonal Antibodies

Anti-NSun2, Polyclonal [EF-1]

Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields
  • Datasheet
  • Inventor Info
Print

Info

Image thumbnail for Anti-NSun2, Polyclonal [EF-1]
Image thumbnail for Anti-NSun2, Polyclonal [EF-1]
Image thumbnail for Anti-NSun2, Polyclonal [EF-1]
Image thumbnail for Anti-NSun2, Polyclonal [EF-1]
Add an image
Primary human keratinocytes: NSun2 (red) DAPI (blue)
Adapted from Frye et al. 2010. Cancer Lett; 289(1):71-80. PMID: 19740597 Figure. Misu protein expression in breast tumours. Immunohistochemistry for Misu (Nsun2) on tissues from breast tumours. Scale bar: 100 μm.
Adapted from Hussain et al. 2009. J Cell Bio; 186(1):27-40. PMID: 19596847 Figure. (A) Misu (green) colocalizes with NPM1 (red) at the nucleolus (arrows) but not at the spindle (arrowheads). (B) In prophase, Misu was found at pericentriolar material (arrowheads) but not the spindle fibers (α-tubulin; arrows). (C and D) In metaphase and anaphase, Misu colocalizes with α-tubulin along the spindle (arrows). (E) In telophase, Misu and α-tubulin localize to the midbody (arrows). DNA is counterstained with DAPI (blue). Bars, 10 µm
Adapted from Frye et al. 2006. Curr Biol; 16(10):971-81. PMID: 16713953 Figure. Western blots showing upregulation of Misu by activation of MycER in human keratinocytes (k) compared to controls (k-pBabe; k-106ER). Actin and tubulin served as loading controls.
Add an image
Image thumbnail for Anti-NSun2, Polyclonal [EF-1] Image thumbnail for Anti-NSun2, Polyclonal [EF-1] Image thumbnail for Anti-NSun2, Polyclonal [EF-1] Image thumbnail for Anti-NSun2, Polyclonal [EF-1]
Catalogue Number 151841
Applications IF IP WB
Antigen/Gene or Protein Targets NSun2 (Misu)
Reactivity Human
Relevance NSun2 is a cytosine-5 RNA methyltransferase of transfer RNAs (tRNAs) and other non-coding RNAs. NSun2 is expressed in various tissues, including skin, brain and testis. Expression of Nsun2 is up-regulated in many tumours.
Host Rabbit
Immunogen NSun2 (Misu) peptides conjugated with keyhole limpet hemocyanin
Formulation Whole serum
Molecular Weight (kDa) 80
Notes Alternative Polyclonal Name: hMetA

Other Research Area: Post-transcriptional modifying enzyme

Note on MW: Mw should be around 80kDa but the band runs around 100kDa in a western

Staining Pattern: Nuclear, mainly in the nucleolus in interphase and at the spindel in M-phase of the cell cycle

Suggested Dilutions: IF: 1:1000; WB: 1:1000; IHC: 1:250
Positive Control Human keratinocytes, human breast cancer cell lines
Research Area Epigenetics & Nuclear Signalling
Immunogen UniProt ID N/A

References

There are 6 reference entries for this reagent.

View All References

References: 6 entries

Frye et al. 2010. Cancer Lett. 289(1):71-80. PMID: 19740597.

View  

Genomic gain of 5p15 leads to over-expression of Misu (NSUN2) in breast cancer.

Europe PMC ID: 19740597

View  

Hussain et al. 2009. J Cell Biol. 186(1):27-40. PMID: 19596847.

View  

The nucleolar RNA methyltransferase Misu (NSun2) is required for mitotic spindle stability.

Europe PMC ID: 19596847

View  

Frye et al. 2006. Curr Biol. 16(10):971-81. PMID: 16713953.

View  

The RNA methyltransferase Misu (NSun2) mediates Myc-induced proliferation and is upregulated in tumors.

Europe PMC ID: 16713953

View  

Add a reference

References: 6 entries

Frye et al. 2010. Cancer Lett. 289(1):71-80. PMID: 19740597.

View  

Genomic gain of 5p15 leads to over-expression of Misu (NSUN2) in breast cancer.

View  

Hussain et al. 2009. J Cell Biol. 186(1):27-40. PMID: 19596847.

View  

The nucleolar RNA methyltransferase Misu (NSun2) is required for mitotic spindle stability.

View  

Frye et al. 2006. Curr Biol. 16(10):971-81. PMID: 16713953.

View  

The RNA methyltransferase Misu (NSun2) mediates Myc-induced proliferation and is upregulated in tumors.

View  

Add a reference

Inventor Information