Anti-ANO5 [5F7]
Invented at University of Durham
- Datasheet
- References (1)
- Inventor Info
Info
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| Catalogue Number | 151849 |
| Applications | IF WB |
| Antigen/Gene or Protein Targets | Anoctamin 5 |
| Reactivity | Human, Mouse and Rat |
| Relevance | Antibody to muscular dystrophy gene. Relevant for anoctamin 5 linked diseases - muscular dystrophy, GDD1, dysferlinopathy. Antibody is of use for diagnostic and research purposes on anoctamin 5 and other anoctamins. The antibody also has relevance in research in cell membrane repair, membrane trafficking and chloride channels. |
| Host | Mouse |
| Immunogen | Sythetic peptide, C-Terminal. "CKREKLMTIKILHDFE" |
| Positive Control | C2C12, skeletal muscle |
| Subclass | IgM |
| Molecular Weight (kDa) | 37 |
| Myeloma Used | Sp2/0-Ag14 |
| Recommended Growing Conditions | DMEM + antibiotic + 10% FCS |
| Notes |
Molecular Weight: The protein has an expected molecular mass of ~107kDa. However, it is rarely detected using this antibody in muscle tissue. By western blot using lysates prepared from C2C12 cells (wt), RD and fibroblasts 3 bands are detected; 37 KDa, 55kDa and the expected molecular mass. In the Aceview database (http://www.ncbi.nlm.nih.gov/IEB/Research/Acembly/av.cgi?db=human&term=ano5&submit=Go) there is evidence from clones of a short C-terminal isoform. This is likely the small isoform detected in control muscle. |
| Research Area | Cardiovascular, Cell Signaling & Signal Transduction |
