Anti-NSE [NSEP2]

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References (2)

Inventor Info

Info

Catalogue Number	151867
Applications	ELISA IHC WB
Antigen/Gene or Protein Targets	Gamma-isozyme of human enolase (NSE)
Reactivity	Human
Relevance	Neuron specific enolase (NSE, or ?-isozyme of enolase) is found at elevated concentrations in plasma in certain neoplasias, including paediatric neuroblastoma and small cell lung cancer.
Host	Mouse
Immunogen	Immunogen: ovalbumin-conjugated synthetic peptides corresponding to human NSE amino acid sequence:NSE-P2: aa’s 271-285 - TGDQLGALYQDFVRD
Positive Control	Formalin-fixed, paraffin-embedded nerve tissue sectionswestern blot: ?-isozyme of human enolase; 50-100 ng per lane
Subclass	IgG1 kappa
Myeloma Used	P3X63Ag8.653
Recommended Growing Conditions	Dulbecco’s media containing 20% Fetal Bovine serum (DH20) prepared as follows (for final volume of 300ml: 237ml DMEM + 60 ml Fetal Bovine Serum plus 3ml L-Glutamine + 3ml of Penicillin -Streptomycin.
Strain	Balb/c
Notes	Not reactive with -isozyme of enolase.
Research Area	Cancer, Cell Type or Organelle Marker, Developmental Biology, Metabolism, Neurobiology, Stem Cell Biology

There are 2 reference entries for this reagent.

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Murray et al. 1993. J Clin Pathol. 46(11):993-6. PMID: 8254105.

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Duncan et al. 1992. J Immunol Methods. 151(1-2):227-36. PMID: 1629611.

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Murray et al. 1993. J Clin Pathol. 46(11):993-6. PMID: 8254105.

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Duncan et al. 1992. J Immunol Methods. 151(1-2):227-36. PMID: 1629611.

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	Ayham Alnabulsi
	from Vertebrate Antibodies Limited Linked to 154 other reagents on Ximbio.

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	Vertebrate Antibodies Limited
	199 reagents listed on Ximbio

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