Anti-PTP1B-OX, Recombinant [scFvs 45]
Invented at Cold Spring Harbor Laboratory
- Datasheet
- References (4)
- Inventor Info
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| Applications | IP Fn |
| Antigen/Gene or Protein Targets | PTP1B |
| Reactivity | Human |
| Relevance |
PTP1B is an intracellular protein tyrosine phosphatase. In its reduced, active state, which is the basal state of the enzyme, PTP1B regulates a number of signalling pathways. For example, PTP1B activity antagonizes insulin signalling and modulates EGF and leptin signaling. PTP1B is indicated in a number of human diseases, such as obesity and diabetes. scFvs 45 is a conformation-sensor antibody, in the form of single chain variable fragments (scFvs), that stabilize PTP1B-oxidised (PTP1B-OX) and thereby inhibit its phosphatase function. Expression of conformation sensor scFvs as intrabodies enhanced insulin-induced tyrosyl phosphorylation of the β-subunit of the insulin receptor and its substrate IRS-1, and increased insulin induced phosphorylation of PKB/AKT. |
| Immunogen | Recombinant protein corresponding to the PTP1B-OX of human PTP1B (Oxidized). |
| Subclass | IgG |
| Research Area | Cardiovascular, Cell Signaling & Signal Transduction |
| Notes |
Host: Chicken Immunogen: Recombinant protein corresponding to human PTP1B-OX |
References: 4 entries
Haque et al. 2012. Nat Protoc. 7(12):2127-43. PMID: 23154784.
The use of phage display to generate conformation-sensor recombinant antibodies.
Europe PMC ID: 23154784
Haque et al. 2011. Cell. 147(1):185-98. PMID: 21962515.
Conformation-sensing antibodies stabilize the oxidized form of PTP1B and inhibit its phosphatase activity.
Europe PMC ID: 21962515
Add a reference
References: 4 entries
Haque et al. 2012. Nat Protoc. 7(12):2127-43. PMID: 23154784.
The use of phage display to generate conformation-sensor recombinant antibodies.
Haque et al. 2011. Cell. 147(1):185-98. PMID: 21962515.
Conformation-sensing antibodies stabilize the oxidized form of PTP1B and inhibit its phosphatase activity.
Add a reference
