Murine Podocyte Cell Line
Invented by Prof Moin Saleem from University Of Bristol
Invented at University Of Bristol
- Datasheet
- References (9)
- Inventor Info
Info
| Host | Mouse |
| Tissue | Kidney |
| Model | Primary line |
| Relevance |
Cell line to study the determinative steps of podocyte differentiation and process formation. Background and Research Application The conditionally immortalised murine podocyte cell line allows an in-vitro process of maturation analogous to the development and maturation of podocytes in-vivo. Both proliferating and differentiating MPC cells express the WT-1 protein and an ordered array of actin fibres and microtubules extends into the forming cellular processes during differentiation, reminiscent of podocyte processes in vivo. The result is a homogenous, stable cell source that shows expression of key antigenic markers of differentiated in-vivo podocytes. These include the novel podocyte proteins nephrin, podocin, CD2AP and synaptopodin. |
| Conditional Description | Immortalization is conditional by use of a temperature-sensitive transgene encoding SV40LT |
| Research Area | Other |
| Recommended Growing Conditions | RPMI-1640, Insulin-Transferrin-Selenium, Foetal Bovine Serum (10% v/v), Pen/Strep medium at at 33°C or 37°C, 5% CO2. |
| Notes |
Points of Interest Applications: Toxicology studies Metabolism Therapeutic targeting Renal physiology R&D Co-culture studies (e.g 3D culture model) Key benefits: Physiologically relevant phenotype analogous to in-vivo differentiated renal cells Homogenous, stable and long lasting Concentration Vial has between 1-5 million cells as standard, however this may vary. |
| Cellosaurus ID | CVCL_AS87 |
References: 9 entries
Peng et al. 2019. Exp Ther Med. 18(6):4829-4837. PMID: 31798708.
Che et al. 2019. PeerJ. 7:e8016. PMID: 31799068.
Khullar et al. 2018. Clin Exp Nephrol. 22(3):508-516. PMID: 29022109.
Bettaieb et al. 2017. Biochim Biophys Acta Gen Subj. 1861(11 Pt A):2758-2765. PMID: 28757338.
Zea et al. 2016. PLoS One. 11(7):e0157907. PMID: 27389192.
Ni et al. 2012. Nephrology (Carlton). 17(6):525-31. PMID: 22591222.
Podocyte culture: tricks of the trade.
Europe PMC ID: 22591222
Mundel et al. 1997. Exp Cell Res. 236(1):248-58. PMID: 9344605.
Rearrangements of the cytoskeleton and cell contacts induce process formation during differentiation of conditionally immortalized mouse podocyte cell lines.
Europe PMC ID: 9344605
Add a reference
References: 9 entries
Peng et al. 2019. Exp Ther Med. 18(6):4829-4837. PMID: 31798708.
Che et al. 2019. PeerJ. 7:e8016. PMID: 31799068.
Khullar et al. 2018. Clin Exp Nephrol. 22(3):508-516. PMID: 29022109.
Bettaieb et al. 2017. Biochim Biophys Acta Gen Subj. 1861(11 Pt A):2758-2765. PMID: 28757338.
Zea et al. 2016. PLoS One. 11(7):e0157907. PMID: 27389192.
Ni et al. 2012. Nephrology (Carlton). 17(6):525-31. PMID: 22591222.
Podocyte culture: tricks of the trade.
Mundel et al. 1997. Exp Cell Res. 236(1):248-58. PMID: 9344605.
Rearrangements of the cytoskeleton and cell contacts induce process formation during differentiation of conditionally immortalized mouse podocyte cell lines.
Add a reference
