Anti-Cytochrome P450 2E1 [M12P4H2]
Invented by Ayham Alnabulsi from Vertebrate Antibodies Limited
Invented at Vertebrate Antibodies Limited
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![Image for Anti-Cytochrome P450 2E1 [M12P4H2]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/e9ccb976-7a33-4911-a37c-fccd61b59f9e.jpg)
![Image for Anti-Cytochrome P450 2E1 [M12P4H2]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/9d4fb36f-b4bb-4b2c-b23f-e82b3ef40487.jpg)
![Immunohistochemistry was performed on formalin-fixed paraffin-embedded normal liver tissue using anti-CYP2E1 antibody [M12P4H2]. Strong cytoplasmic immunostaining was observed in agreement with existing literature. No staining was detected in other tissue types (data not shown). Antigen retrieval step was required (microwave 10 min @ 950W in 0.01M sodium citrate buffer, pH 6.0).](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/0d72ffca-fcd8-487b-9d84-73bc20f7a672.jpg)
![Image for Anti-Cytochrome P450 2E1 [M12P4H2]](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/e9ccb976-7a33-4911-a37c-fccd61b59f9e.jpg)
![Image for Anti-Cytochrome P450 2E1 [M12P4H2]](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/9d4fb36f-b4bb-4b2c-b23f-e82b3ef40487.jpg)
![Immunohistochemistry was performed on formalin-fixed paraffin-embedded normal liver tissue using anti-CYP2E1 antibody [M12P4H2]. Strong cytoplasmic immunostaining was observed in agreement with existing literature. No staining was detected in other tissue types (data not shown). Antigen retrieval step was required (microwave 10 min @ 950W in 0.01M sodium citrate buffer, pH 6.0).](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/0d72ffca-fcd8-487b-9d84-73bc20f7a672.jpg)
![Image thumbnail for Anti-Cytochrome P450 2E1 [M12P4H2]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/e9ccb976-7a33-4911-a37c-fccd61b59f9e.jpg)
![Image thumbnail for Anti-Cytochrome P450 2E1 [M12P4H2]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/9d4fb36f-b4bb-4b2c-b23f-e82b3ef40487.jpg)
![Image thumbnail for Anti-Cytochrome P450 2E1 [M12P4H2]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/0d72ffca-fcd8-487b-9d84-73bc20f7a672.jpg)
| Catalogue Number | 152160 |
| Applications | IHC WB |
| Antigen/Gene or Protein Targets | Cytochrome P450, family 2, subfamily E, polypeptide 1 (CYP2E1) |
| Reactivity | Human |
| Relevance | Metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. Inactivates a number of drugs and xenobiotics and also bioactivates many xenobiotic substrates to their hepatotoxic or carcinogenic forms. |
| Host | Mouse |
| Immunogen | Ovalbumin-conjugated synthetic peptide HIGFGCIPPR (C-terminal sequence) |
| Positive Control | IHC: formalin-fixed, paraffin-embedded human liver sections; WB: pooled human liver microsomes |
| Subclass | IgG1 lambda |
| Myeloma Used | P3X63Ag8.653 |
| Recommended Growing Conditions | Dulbecco’s media containing 20% Fetal Bovine serum (DH20) prepared as follows (for final volume of 300ml: 237ml DMEM plus 60 ml Fetal Bovine Serum plus 3ml L-Glutamine). |
| Strain | Balb/c |
| Research Area | Cancer, Cardiovascular, Cell Signaling & Signal Transduction, Metabolism |
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