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Recombinant Antibodies

Anti-EGFR, Recombinant [EGFR1]

Invented by Dr Peter Goodfellow from Abingworth Life Sciences
Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields
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Catalogue Number 152583
Applications FACS IHC IF IP WB
Antigen/Gene or Protein Targets Epidermal Growth Factor Receptor (EGFR, Her1)
Reactivity Human
Relevance Recombinant antibody which binds EGFR1 tyrosine kinase, commonly mutated in a range of cancers.

Background and Research Application
The EGFR family of type I growth factor receptor tyrosine kinases includes EGFR (HER1), c-erbB2 (HER2; neu), c-erbB3 (HER3) and c-erbB4 (HER4). EGFR activation signals multiple downstream signalling cascade pathways such as the Ras - ERK, PI3-K - Akt, Jak - STAT and PKC pathways which help in growth and proliferation of cells. Dysregulation of EGFR signalling as a consequence of overexpression, amplification and mutation of the EGFR gene occurs frequently in several types of cancers, including head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, oesophagus, stomach and in squamous cell carcinoma. Tumours become dependent on EGFR signalling to maintain their malignant phenotypes.
This is a recombinant version of the anti-EGFR1 monoclonal antibody.
Host Mouse
Immunogen Human epidermoid carcinoma line A431
Subclass IgG2b
Formulation PBS only
Concentration 1mg/ml
Molecular Weight (kDa) 175
Research Area Cancer, Cell Signaling & Signal Transduction
Immunogen UniProt ID P00533
Notes Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Points of Interest
Anti-EGFR1 has no effect on tyrosine kinase activity of EGFR, and does not react with erbB-2, erbB-3 and erbB-4.

Recombinant monoclonal antibody produced from the original monoclonal. Manufactured using Absolute Antibody’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma.

Concentration
1mg/ml as standard

References

There are 2 reference entries for this reagent.

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References: 2 entries

Original hybridoma first published in Waterfield et al. 1982. J Cell Biochem. 20(2):149-61. PMID: 6188757.

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A monoclonal antibody to the human epidermal growth factor receptor.

Europe PMC ID: 6188757

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References: 2 entries

Original hybridoma first published in Waterfield et al. 1982. J Cell Biochem. 20(2):149-61. PMID: 6188757.

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A monoclonal antibody to the human epidermal growth factor receptor.

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Inventor Information