Anti-Involucrin [SY5]
Invented by Prof Fiona Watt at Cancer Research UK London Research Institute: Lincoln's Inn Fields
Anti-Involucrin, Recombinant [SY5]
Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields
- Datasheet
- References (2)
- Inventor Info
Info
| Catalogue Number | 152587 |
| Applications | IHC IF IP WB |
| Antigen/Gene or Protein Targets | Involucrin |
| Reactivity | Dog, Human, Pig and Primate |
| Relevance | Clone SY5 recognizes a protein of 66kDa-170kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this MAb reacts with a 120kDa protein. It stains the involucrin in a variety of sizes: 170kDa in MCF-7 cells, a doublet of ~115kDa and 150kDa in gorilla and owl monkey, 66kDa in dog, and a doublet of 105kDa in pig. Its epitope maps between codon 421-568 of human involucrin. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it.Involucrin is a protein precursor of the epidermal cornified envelope and a differentiation marker of human keratinocytes. SY5 is useful for studies of changes in involucrin expression in pathological studies. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal; expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix. |
| Host | Mouse |
| Immunogen | Purified involucrin from human keratinocytes. |
| Subclass | IgG1 |
| Formulation | PBS only |
| Concentration | 1mg/ml |
| Research Area | Cancer, Cell Signaling & Signal Transduction |
| Notes |
There is weak reactivity with Canine Recombinant monoclonal antibody produced from the original monoclonal. Manufactured using Absolute Antibody’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma. |
References: 2 entries
Original hybridoma first published in Hudson et al. 1992. Hybridoma. 11(3):367-79. PMID: 1500072.
Characterisation of eight monoclonal antibodies to involucrin.
Europe PMC ID: 1500072
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References: 2 entries
Original hybridoma first published in Hudson et al. 1992. Hybridoma. 11(3):367-79. PMID: 1500072.
Characterisation of eight monoclonal antibodies to involucrin.
Add a reference
