SVCT Cell Line
Invented by Dr Sidney Chang at Marie Curie Research Institute
SVCT-MI2 Cell Line
Invented by Prof Joyce Taylor-Papadimitriou from King's College London , Dr Sidney Chang from Retired Consultant
Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields
- Datasheet
- References (2)
- Inventor Info
Info
| Catalogue Number | 152596 |
| Antigen/Gene or Protein Targets | Human oncogene EJ Harvey-ras |
| Host | Human |
| Tissue | Breast |
| Disease Keywords | Breast Cancer |
| Relevance | SVCT-MI2 was established in 1987 from epithelial cells in breast milk secretions. The cells have been transfected by microinjection using SV40 DNA and a plasmid containing the neomycin gene and the human oncogene EJ Harvey-ras. After selection of G418-resistant colonies, the clone SVCT-MI2 was shown to be tumorigenic in nude mice. They also have the ability to grow anchorage-independent if cultured in soft agar. |
| Conditional | No |
| Research Area | Cancer, Cell Cycle, Drug Discovery & Development, Epigenetics & Nuclear Signalling |
| Growth/Phenotype Keywords | Adherent |
| Recommended Growing Conditions | RPMI 1640 or DMEM + 2mM Glutamine + 10µg / ml insulin + 5µg / ml hydrocortisone + 10% FBS. Subculture routine: Split sub-confluent cultures (70-80%) 1:4 to 1:10 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Note: cells may pile up when confluent. |
| Cellosaurus ID | CVCL_2719 |
References: 2 entries
Yamashita et al. 2009. Bioorg Med Chem. 17(17):6286-91. PMID: 19674905.
Synthesis and evaluation of bioactive naphthoquinones from the Brazilian medicinal plant, Tabebuia avellanedae.
Europe PMC ID: 19674905
Add a reference
References: 2 entries
Yamashita et al. 2009. Bioorg Med Chem. 17(17):6286-91. PMID: 19674905.
Synthesis and evaluation of bioactive naphthoquinones from the Brazilian medicinal plant, Tabebuia avellanedae.
Add a reference
Inventor Information
Inventors
|
Dr Sidney Chang |
