E3 Cell Line
Invented by Prof Joyce Taylor-Papadimitriou from King's College London , Prof Joy Burchell from King's College London
Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields
- Datasheet
- References (6)
- Inventor Info
Info
| Catalogue Number | 152597 |
| Antigen/Gene or Protein Targets | hMUC1, hygromycin resistance gene |
| Parental Line | 410.4 |
| Host | Mouse |
| Tissue | Breast |
| Disease Keywords | Immunological diseases; mammary cancer |
| Model | Immortalised Line |
| Relevance | The E3 cell line was developed to examine the possibility of using the MUC1 gene and its products in active immunization against breast and other carcinomas. The human MUC1 gene codes for a type I membrane glycoprotein that is normally expressed on the apical surface of most glandular epithelial cells, but which is upregulated and under- or differently glycosylated in carcinomas; these differences in glycosylation lead to the exposure of novel epitopes which are not found on the normally processed mucin and can therefore be used as targets. |
| Production Details | The E3 cell line was developed from the 410.4 murine mammary cell line by transfection of the hygromycin resistance gene with the MUC1 gene. |
| Research Area | Cancer, Drug Discovery & Development, Immunology |
| Recommended Growing Conditions | DMEM, 10%FCS , 400µg/ml hygromycin |
| Cellosaurus ID | CVCL_W345 |
References: 6 entries
Julien et al. 2009. Br J Cancer. 100(11):1746-54. PMID: 19436292.
Sialyl-Tn vaccine induces antibody-mediated tumour protection in a relevant murine model.
Europe PMC ID: 19436292
Smith et al. 1999. Immunology. 97(4):648-55. PMID: 10457219.
Expression of B7.1 in a MUC1-expressing mouse mammary epithelial tumour cell line inhibits tumorigenicity but does not induce autoimmunity in MUC1 transgenic mice.
Europe PMC ID: 10457219
Lalani et al. 1991. J Biol Chem. 266(23):15420-6. PMID: 1714457.
Expression of the gene coding for a human mucin in mouse mammary tumor cells can affect their tumorigenicity.
Europe PMC ID: 1714457
Add a reference
References: 6 entries
Julien et al. 2009. Br J Cancer. 100(11):1746-54. PMID: 19436292.
Sialyl-Tn vaccine induces antibody-mediated tumour protection in a relevant murine model.
Smith et al. 1999. Immunology. 97(4):648-55. PMID: 10457219.
Expression of B7.1 in a MUC1-expressing mouse mammary epithelial tumour cell line inhibits tumorigenicity but does not induce autoimmunity in MUC1 transgenic mice.
Lalani et al. 1991. J Biol Chem. 266(23):15420-6. PMID: 1714457.
Expression of the gene coding for a human mucin in mouse mammary tumor cells can affect their tumorigenicity.
Add a reference
