CMT 64/61 Cell Line
Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields
- Datasheet
- References (2)
- Inventor Info
Info
| Catalogue Number | 152751 |
| Host | Mouse |
| Tissue | Lung |
| Disease Keywords | Lung carcinoma, metastatic |
| Model | Tumour line |
| Relevance | The tumourigenic and metastatic CMT 64/61 murine cell line is derived from an alveologenic lung carcinoma of a C57BL/1CRF mouse. It can be maintained in a completely defined serum free medium, but the growth rate is much slower than in supplemented serum. It has been used in studies of lung metastases. |
| Production Details | Mouse; CMT 64 isolated from primary alveogenic lung carcinoma tumour mass in C57BL/lcrf mouse, achieving stable morphology and growth rate in culture, similar to growth rate and morphology in primary tumour, and in lung metastasis induced after subcutaneous innoculation of mice. |
| Conditional | No |
| Research Area | Cancer, Drug Discovery & Development |
| Growth/Phenotype Keywords | Tumorigenesis, adherent |
| Recommended Growing Conditions | Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Culture Medium: Waymouth's MB 752/1 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). |
| Cellosaurus ID | CVCL_4146 |
References: 2 entries
Franks et al. 1976. Cancer Res. 36(3):1049-55. PMID: 1253168.
Metastasizing tumors from serum-supplemented and serum-free cell lines from a C57BL mouse lung tumor.
Europe PMC ID: 1253168
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References: 2 entries
Franks et al. 1976. Cancer Res. 36(3):1049-55. PMID: 1253168.
Metastasizing tumors from serum-supplemented and serum-free cell lines from a C57BL mouse lung tumor.
Add a reference
