HeLa mCherry-Histone H2B EGFP-Alpha Tubulin Cell Line
Invented by Prof Francis Barr from University of Oxford
Invented at University of Liverpool
- Datasheet
- References (6)
- Inventor Info
Info
| Catalogue Number | 152987 |
| Antigen/Gene or Protein Targets | Histone H2B, Alpha Tubulin |
| Parental Line | HeLa |
| Host | Human |
| Tissue | Cervix |
| Model | Reporter |
| Relevance | The human histone H2B gene was fused to the gene encoding mCherry and Alpha Tubulin was similarly fused to EGFP. Both constructs were transfected into human HeLa cells to generate a stable line constitutively expressing H2B-mCherry and EGFP-Alpha Tubulin. The mCherry-Histone H2B fusion protein was incorporated into nucleosomes without affecting cell cycle progression. The cell line allows high-resolution imaging of both mitotic chromosomes and interphase chromatin. |
| Research Area | Cell Cycle, Cell Type or Organelle Marker, Cell Structure and Motility, DNA Damage and Repair, Genetic Studies Tools |
| Recommended Growing Conditions | DMEM, 10% FBS, 5% CO2, 37oC. Antibiotic selection for GFP and mCherry expression: 1μg/mL Puromycin, 2μg/mL Blasticidine, expression is quite stable but selecting at least every two passages is recommended. |
References: 6 entries
Dunsch et al. 2012. J Cell Biol. 198(6):1039-54. PMID: 22965910.
Dynein light chain 1 and a spindle-associated adaptor promote dynein asymmetry and spindle orientation.
Europe PMC ID: 22965910
Zeng et al. 2010. J Cell Biol. 191(7):1315-32. PMID: 21187329.
Protein phosphatase 6 regulates mitotic spindle formation by controlling the T-loop phosphorylation state of Aurora A bound to its activator TPX2.
Europe PMC ID: 21187329
Bastos et al. 2010. J Cell Biol. 191(4):751-60. PMID: 21079244.
Plk1 negatively regulates Cep55 recruitment to the midbody to ensure orderly abscission.
Europe PMC ID: 21079244
Add a reference
References: 6 entries
Dunsch et al. 2012. J Cell Biol. 198(6):1039-54. PMID: 22965910.
Dynein light chain 1 and a spindle-associated adaptor promote dynein asymmetry and spindle orientation.
Zeng et al. 2010. J Cell Biol. 191(7):1315-32. PMID: 21187329.
Protein phosphatase 6 regulates mitotic spindle formation by controlling the T-loop phosphorylation state of Aurora A bound to its activator TPX2.
Bastos et al. 2010. J Cell Biol. 191(4):751-60. PMID: 21079244.
Plk1 negatively regulates Cep55 recruitment to the midbody to ensure orderly abscission.
Add a reference
