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Cell Lines

T47D-E2J Cell Line

Invented by Prof Joy Burchell from King's College London , Prof Joyce Taylor-Papadimitriou from King's College London
Invented at King's College London
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Catalogue Number 153169
Antigen/Gene or Protein Targets Glycosyltransferase - C2GnT1
Parental Line T47D
Synonyms C2GnT
C2GnT-L
Host Human
Tissue Breast
Disease Keywords Breast Cancer
Relevance The parental T47D cell line was established from the pleural effusion of a ductal carcinoma of the breast. The tumour cell line, T47D-E2J, was created as a breast cancer model with an O-linked glycosylation profile similar to normal mammary epithelial cells. T47D-E2J cells overexpress the enzyme C2GnT1, a beta-1,3galactosyl-O-glycosyl-glycoprotein.

C2GnT1 is the dominant enzyme expressed in normal breast tissue known to convert core 1 glycoproteins to core 2 glycoproteins, by the addition of N-acetylglucosamine to N-acetylgalactosamine. Expression of the C2GnT1 enzyme has been found to be decreased or absent in most breast cancer cell lines despite still expressing mRNAs coding for this enzyme.

Together with wild-type T47D and T47D-STn these cell lines represent a panel of isogenic cells with different O-linked glycosylation patterns.
Conditional No
Research Area Cancer, Drug Discovery & Development
Recommended Growing Conditions DMEM 10% FCS and 500µg/ml G418

References

There are 4 reference entries for this reagent.

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References: 4 entries

Sewell et al. 2006. J Biol Chem. 281(6):3586-94. PMID: 16319059.

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The ST6GalNAc-I sialyltransferase localizes throughout the Golgi and is responsible for the synthesis of the tumor-associated sialyl-Tn O-glycan in human breast cancer.

Europe PMC ID: 16319059

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Dalziel et al. 2001. J Biol Chem. 276(14):11007-15. PMID: 11118434.

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The relative activities of the C2GnT1 and ST3Gal-I glycosyltransferases determine O-glycan structure and expression of a tumor-associated epitope on MUC1.

Europe PMC ID: 11118434

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References: 4 entries

Sewell et al. 2006. J Biol Chem. 281(6):3586-94. PMID: 16319059.

View  

The ST6GalNAc-I sialyltransferase localizes throughout the Golgi and is responsible for the synthesis of the tumor-associated sialyl-Tn O-glycan in human breast cancer.

View  

Dalziel et al. 2001. J Biol Chem. 276(14):11007-15. PMID: 11118434.

View  

The relative activities of the C2GnT1 and ST3Gal-I glycosyltransferases determine O-glycan structure and expression of a tumor-associated epitope on MUC1.

View  

Add a reference

Inventor Information