Anti-C-NAP1/CEP250 [6F2C8]
Invented by Prof Ciaran Morrison from National University of Ireland, Galway
Invented at National University of Ireland, Galway , Centre For Chromosome Biology
- Datasheet
- References (2)
- Inventor Info
Info
| Catalogue Number | 153171 |
| Applications | IHC IP WB |
| Antigen/Gene or Protein Targets | Centrosomal Nek2-Associated Protein 1 |
| Synonyms | Centrosome-associated protein CEP250; Centrosomal Protein 250; Centrosomal Protein 250kDa; Centrosomal Nek2-Associated Protein 1; 250 KDa Centrosomal Protein; Centrosomal Protein 2; C-NAP1; CNAP1 |
| Reactivity | Human |
| Relevance | Centrosomal Protein 250 (CEP250), also known as Centrosomal Nek2-Associated Protein 1 (C-NAP1), is a core centrosomal protein required for centriole-centriole cohesion during interphase of the cell cycle. CEP250 dissociates from the centrosomes when parental centrioles separate at the beginning of mitosis. The protein associates with and is phosphorylated by NIMA-related kinase 2, which is also associated with the centrosome. |
| Host | Mouse |
| Immunogen | Recombinant human C-NAP1 amino acids 1513-1750 (bacterially expressed from pGEX4T as a GST fusion, then thrombin-cleaved and purified). |
| Positive Control | Immunofluorescence and Western Blot: Jurkat cells, hTERT-RPE1 cells, Immunofluorescence only: PC3 cells, DU145 cells, PWR1E cells, Peripheral Blood Mononuclear Cells (PBMCs) |
| Subclass | IgG1 kappa |
| Molecular Weight (kDa) | 250 |
| Myeloma Used | Sp2/0-Ag14 |
| Recommended Growing Conditions | DMEM + 10% FBS + hypoxanthine supplement + non-essential amino acids |
| Research Area | Cell Cycle, Immunology |
References: 2 entries
Flanagan et al. 2017. Mol Biol Cell. :. PMID: 28100636.
IHC WB
Centriole splitting caused by loss of the centrosomal linker protein C-NAP1 reduces centriolar satellite density and impedes centrosome amplification.
Europe PMC ID: 28100636
Add a reference
References: 2 entries
Flanagan et al. 2017. Mol Biol Cell. :. PMID: 28100636.
IHC WB
Centriole splitting caused by loss of the centrosomal linker protein C-NAP1 reduces centriolar satellite density and impedes centrosome amplification.
Add a reference
