HCT 116 EphA2 KO Tet-inducible Cell Line
Invented at Queen's University Belfast
- Datasheet
- References (2)
- Inventor Info
Info
| Catalogue Number | 153207 |
| Antigen/Gene or Protein Targets | EphA2 |
| Parental Line | HCT 116 |
| Host | Human |
| Tissue | Colon |
| Disease Keywords | Colorectal cancer |
| Model | Knock-Out |
| Relevance | EphA2 is an important regulator of tumour initiation, neo-vascularization and metastasis in a wide range of cancers. The HCT 116 EphA2 KO Tet-inducible cell line was developed to address the involvement of EphA2 where it was shown that silencing of the protein suppressed migration and invasion. |
| Production Details | HCT 116 cells were transfected with pTRIPZ plasmid encoding Tet-inducible shRNA against EphA2. Stably transfected cells were selected, maintained in mediumsupplemented with 0.5μg/mL puromycin and induced with 2μg/ml doxycycline. |
| Conditional | Yes |
| Conditional Description | Conditional knockdown of endogenous EphA2 expression upon treatment with Tetracycline |
| Research Area | Cancer, Cell Structure and Motility, Drug Discovery & Development |
| Growth/Phenotype Keywords | Invasion, migration |
| Recommended Growing Conditions | McCoy’s 5a Medium (GIBCO # 16600) + 10% FBS + 100 units/ml penicillin+ 100 μg/ml streptomycin |
| Cellosaurus ID | CVCL_HG05 |
References: 2 entries
Dunne et al. 2016. Clin Cancer Res. 22(1):230-42. PMID: 26283684.
EphA2 Expression Is a Key Driver of Migration and Invasion and a Poor Prognostic Marker in Colorectal Cancer.
Europe PMC ID: 26283684
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References: 2 entries
Dunne et al. 2016. Clin Cancer Res. 22(1):230-42. PMID: 26283684.
EphA2 Expression Is a Key Driver of Migration and Invasion and a Poor Prognostic Marker in Colorectal Cancer.
Add a reference
