fR2 Cell Line
Invented by Prof Joyce Taylor-Papadimitriou from King's College London , Dr Sidney Chang from Retired Consultant
Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields
- Datasheet
- References (2)
- Inventor Info
Info
| Catalogue Number | 153246 |
| Host | Human |
| Tissue | Breast |
| Disease Keywords | Breast cancer |
| Model | Immortalised Line |
| Relevance | fR2 cells have been shown to express Muc-1, also known as HMFG-1 antigen, which is a mucin-like component of human milk fat globule membranes. They are positive for keratin 8 and 18, but negative for keratins 4, 6, 7, 10, 13, 14, 16, 17 and 19. Although fR2 is morphologically different from fR5, they have probably been derived from a common precursor cell since the karyotypes are similar. Karyotype analysis revealed hypotetraploidy and several rearrangements involving chromosome 1 and 11 which are frequently found in breast carcinomas and lines derived from metastatic pleural effusions. fR2 cells display anchorage independent growth in soft agar and are positive for SV40 T-antigen. Early passages were non-tumorigenic in nude mice. |
| Production Details | The breast epithelial cell line fR2 was established in 1982 by infecting suspensions of primary milk cultures with wild-type SV40. |
| Conditional | No |
| Research Area | Cancer, Drug Discovery & Development |
| Growth/Phenotype Keywords | Adherent |
| Recommended Growing Conditions | Split sub-confluent cultures (70-80%) 1:10 i.e. seeding 1 x 10,000 cells / cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Culture Medium: RPMI 1640 + 2mM Glutamine + 10µg/ml insulin + 5µg/ml hydrocortisone + 10% FBS |
| Notes |
STR-PCR Data: Amelogenin: X CSF1PO: 12,13 D13S317: 12 D16S539: 11,13 D5S818: 12,13 D7S820: 8,12 THO1: 9.3 TPOX: 8 vWA: 20 |
| Cellosaurus ID | CVCL_2444 |
References: 2 entries
Chang et al. 1982. Cancer Res. 42(5):2040-53. PMID: 6279290.
Establishment and characterization of SV40-transformed human breast epithelial cell lines.
Europe PMC ID: 6279290
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References: 2 entries
Chang et al. 1982. Cancer Res. 42(5):2040-53. PMID: 6279290.
Establishment and characterization of SV40-transformed human breast epithelial cell lines.
Add a reference
Inventor Information
Inventors
|
Dr Sidney Chang |
