fR5 Cell Line
Invented by Prof Joyce Taylor-Papadimitriou from King's College London , Dr Sidney Chang from Retired Consultant
Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields
- Datasheet
- References (2)
- Inventor Info
Info
| Catalogue Number | 153247 |
| Host | Human |
| Tissue | Breast |
| Disease Keywords | Breast cancer |
| Model | Immortalised Line |
| Relevance | fR5 cells do not express Muc-1, also known as HMFG-1 antigen, which is a mucin-like component of human milk fat globule membranes. They are negative for keratins 4, 6, 7, 8, 10, 13 14, 16, 17, 18 and 19. Although fR5 is morphologically different from fR2, they have probably been derived from a common precursor cell since the karyotypes are similar. Karyotype analysis revealed hypotetraploidy and several rearrangements involving chromosome 1 and 11 which are frequently found in breast carcinomas and lines derived from metastatic pleural effusions. fR5 cells display anchorage independent growth in soft agar and are positive for SV40 T-antigen. Early passages were non-tumourigenic in nude mice. |
| Production Details | The breast epithelial cell line fR5 was established in 1982 by infecting suspensions of primary milk cultures with wild-type SV40. |
| Conditional | No |
| Research Area | Cancer, Drug Discovery & Development |
| Growth/Phenotype Keywords | Adherent |
| Recommended Growing Conditions | Split sub-confluent cultures (70-80%) 1:10 i.e. seeding 1 x 10,000 cells / cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Culture Medium: RPMI 1640 + 2mM Glutamine + 10µg/ml insulin + 5µg/ml hydrocortisone + 10% FBS |
| Cellosaurus ID | CVCL_2445 |
References: 2 entries
Chang et al. 1982. Cancer Res. 42(5):2040-53. PMID: 6279290.
Establishment and characterization of SV40-transformed human breast epithelial cell lines.
Europe PMC ID: 6279290
Add a reference
References: 2 entries
Chang et al. 1982. Cancer Res. 42(5):2040-53. PMID: 6279290.
Establishment and characterization of SV40-transformed human breast epithelial cell lines.
Add a reference
Inventor Information
Inventors
|
Dr Sidney Chang |
