3.334 SC5/8 Cell Line
Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields
- Datasheet
- References (2)
- Inventor Info
Info
| Catalogue Number | 153249 |
| Parental Line | 3T3TK3 cells and normal rat kidney cells |
| Host | Hybrid |
| Tissue | Fibroblast |
| Model | Tumour line |
| Relevance | Hybrid between un-transformed mouse 3T3TK3 cells and normal rat kidney cells transformed with RSV. The hybrid cells express a non-transformed phenotype. The cells exhibit anchorage-independent growth at 35°C but not at 39.5°C, the non-permissive temperature and showed differences in microfilament bundle formation at both temperatures. |
| Conditional | No |
| Research Area | Adhesion, Cell Structure and Motility |
| Growth/Phenotype Keywords | Adherent |
| Recommended Growing Conditions | Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 35°C; 5% CO2.Culture Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). |
| Notes | Rat x Mouse hybrid |
| Cellosaurus ID | CVCL_7237 |
References: 2 entries
Marshall et al. 1980. Exp Cell Res. 127(2):373-84. PMID: 6247175.
Suppression of the transformed phenotype with retention of the viral 'src' gene in cell hybrids between Rous sarcoma virus-transformed rat cells and untransformed mouse cells.
Europe PMC ID: 6247175
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References: 2 entries
Marshall et al. 1980. Exp Cell Res. 127(2):373-84. PMID: 6247175.
Suppression of the transformed phenotype with retention of the viral 'src' gene in cell hybrids between Rous sarcoma virus-transformed rat cells and untransformed mouse cells.
Add a reference
