RT112/84 Cell Line
Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields
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Info
| Catalogue Number | 153250 |
| Host | Human |
| Tissue | Bladder |
| Disease Keywords | Human bladder carcinoma epithelial |
| Model | Tumour line |
| Relevance | A slow growing tumour which does not form colonies in soft agar. Tumorigenic in nude mice, RT1112/84 cells possess a low plating efficiency requiring 400 cells/dish to form any colonies. |
| Conditional | No |
| Research Area | Cancer, Drug Discovery & Development |
| Growth/Phenotype Keywords | Adherent |
| Recommended Growing Conditions | Subculture Routine: Split sub-confluent cultures (70-80%) seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO2; 37°C. Subculture weekly - cells are difficult to trypsinise, and should be kept sub-confluent.Culture Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS). |
| Notes |
STR-PCR Data: Amelogenin: X CSF1PO: 10,11 D13S317: 13,14 D16S539: 11,13 D5S818: 10,13 D7S820: 11,12 THO1: 7 TPOX: 8,11 vWA: 14,17 |
| Cellosaurus ID | CVCL_2714 |
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