DOK Cell Line
Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields
- Datasheet
- References (4)
- Inventor Info
Info
| Catalogue Number | 153251 |
| Host | Human |
| Tissue | Tongue |
| Disease Keywords | Human Caucasian dysplastic oral keratinocyte |
| Model | Tumour line |
| Relevance | The degree of dysplasia in the patient was described as mild to moderate. The cells show some stratification in confluent cultures and contain a keratin profile similar to the original dysplasia. Growth is stimulated by hydrocortisone and inhibited by cholera toxin. Expression of p53 is increased and a 12 bp in-frame deletion of the p53 gene (codon 188-191) could be identified. DOK cells are non-tumourigenic in athymic nude mice. |
| Production Details | The dysplastic oral keratinocyte cell line DOK was isolated from a piece of dorsal tongue from a 57 year old man. After a squamous-cell carcinoma was removed from the patient, who had been a heavy smoker, the remaining dysplasia was removed subsequently and used to initiate primary cultures leading to the establishment of DOK. |
| Conditional | No |
| Research Area | Cancer, Drug Discovery & Development |
| Growth/Phenotype Keywords | Adherent |
| Recommended Growing Conditions | Subculture Routine: Split sub-confluent cultures (70-80%) 1:10 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.Culture Medium: DMEM + 2mM Glutamine + 5µg/ml Hydrocortisone + 10% Foetal Bovine Serum (FBS). |
| Notes |
STR-PCR Data: Amelogenin: X,Y CSF1PO: 12 D13S317: 11,13 D16S539: 9,12 D5S818: 12 D7S820: 11,13 THO1: 6,8 TPOX: 10 vWA: 14,18 |
| Cellosaurus ID | CVCL_1180 |
References: 4 entries
Burns et al. 1994. Br J Cancer. 70(4):591-5. PMID: 7917902.
The p53 status of cultured human premalignant oral keratinocytes.
Europe PMC ID: 7917902
Chang et al. 1992. Int J Cancer. 52(6):896-902. PMID: 1459732.
DOK, a cell line established from human dysplastic oral mucosa, shows a partially transformed non-malignant phenotype.
Europe PMC ID: 1459732
Add a reference
References: 4 entries
Burns et al. 1994. Br J Cancer. 70(4):591-5. PMID: 7917902.
The p53 status of cultured human premalignant oral keratinocytes.
Chang et al. 1992. Int J Cancer. 52(6):896-902. PMID: 1459732.
DOK, a cell line established from human dysplastic oral mucosa, shows a partially transformed non-malignant phenotype.
Add a reference
