Anti-5-Hydroxymethylcytosine (5hmc), Recombinant [AB3/63.3]
Invented at Babraham Institute
- Datasheet
- References (1)
- Inventor Info
Info
| Catalogue Number | 153274 |
| Applications | IF IP DB |
| Antigen/Gene or Protein Targets | 5-Hydroxymethylcytosine (5hmC) |
| Reactivity | All |
| Relevance | The anti-5-Hydroxymethylcytosine antibody, clone AB3/63.3, detects 5-hydroxymethyl cytosine (hmc, 5hmc), but not 5-methylcytosine or unmethylated cytosine. 5-Hydroxymethylcytosine is a DNA pyrimidine nitrogen base. It is formed from the DNA base cytosine by adding a methyl group and then a hydroxy group. Every mammalian cell contains 5-hydroxymethylcytosine, but the levels vary depending on the cell type; generally, the levels of 5-hydroxymethylcytosine increase with age. Although the exact function has not been fully elucidated, studies suggest that 5-hydroxymethylcytosine may regulate gene expression or initiate DNA demethylation. |
| Host | Rat |
| Immunogen | 5-Hydroxymethylcytosine conjugated to BSA |
| Subclass | IgG2a kappa |
| Formulation | PBS |
| Concentration | 1mgml-1 |
| Positive Control | Unmodified DNA, 5-Methylcytosine DNA, and 5-hydroxymethylcytosine DNA |
| Research Area | Epigenetics & Nuclear Signalling |
| Notes |
Recombinant monoclonal antibody produced from the original monoclonal cell line. Manufactured using Absolute Antibody’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma. Three products based on this clone: - Anti-5-Hydroxymethylcytosine [AB3/63.3], Rat IgG2a, Kappa - Anti-5-Hydroxymethylcytosine [AB3/63.3], Rat IgG2a, Kappa, engineered Fc domain containing key point mutations that abrogate binding to Fc gamma receptors - Anti-5-Hydroxymethylcytosine [AB3/63.3], Rabbit IgG, Kappa |
References: 1 entry
Original hybridoma first published in: Wossidlo et al. 2011. Nat Commun. 2:241. PMID: 21407207.
Add a reference
References: 1 entry
Original hybridoma first published in: Wossidlo et al. 2011. Nat Commun. 2:241. PMID: 21407207.
Add a reference
