Anti-Mycobacterium bovis [11G3]
Invented by Dr Irene Grant from Queen's University Belfast , Dr Linda Stewart from Queen's University Belfast
Invented at Queen's University Belfast
- Datasheet
- References (4)
- Inventor Info
Info
| Catalogue Number | 153320 |
| Applications | ELISA IP |
| Antigen/Gene or Protein Targets | Mycobacterium bovis |
| Reactivity | Mycobacterium bovis |
| Relevance | Bovine tuberculosis (TB) caused by Mycobacterium bovis continues to be a significant animal health issue in some countries, including the United Kingdom and Ireland. Anti-Mycobacterium bovis [11G3] has been demonstrated to work by ELISA and for immunocapture. |
| Host | Mouse |
| Immunogen | Gamma-irradiated whole M. bovis AF2122/97 cells |
| Positive Control | M. bovis AF2122/97 cells |
| Subclass | IgM |
| Myeloma Used | Sp2/0-Ag14 |
| Recommended Growing Conditions | DMEM containing 10% (v/v) fetal bovine serum and 5% (v/v) penicillin/streptomycin |
| Strain | Balb/c |
| Notes |
Direct and competitive ELISA. Specificity of immunocapture was evaluated in Stewart et al., 2012. "MAb 11G3-coated beads and beads coated with a mixture of MAb 11G3 and biotinylated peptide EEA302 yielded PCR bands of decreasing intensity across all five cell concentrations tested and thus demonstrated the best M. bovis capture capability". |
| Research Area | Bacteriology |
References: 4 entries
Improved detection of Mycobacterium bovis infection in bovine lymph node tissue using immunomagnetic separation (IMS)-based methods.
Europe PMC ID: 23469275
Stewart et al. 2013. PLoS One. 8(3):e58374. PMID: 23469275.
Stewart et al. 2012. J Clin Microbiol. 50(5):1598-605. PMID: 22322353.
Production and evaluation of antibodies and phage display-derived peptide ligands for immunomagnetic separation of Mycobacterium bovis.
Europe PMC ID: 22322353
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References: 4 entries
Improved detection of Mycobacterium bovis infection in bovine lymph node tissue using immunomagnetic separation (IMS)-based methods.
Stewart et al. 2013. PLoS One. 8(3):e58374. PMID: 23469275.
Stewart et al. 2012. J Clin Microbiol. 50(5):1598-605. PMID: 22322353.
Production and evaluation of antibodies and phage display-derived peptide ligands for immunomagnetic separation of Mycobacterium bovis.
Add a reference
