H103 Cell Line
Invented at University Of Bristol
- Datasheet
- References (10)
- Inventor Info
Info
| Catalogue Number | 153419 |
| Antigen/Gene or Protein Targets | Human oral squamous cell carcinoma, tongue |
| Tissue | Tongue |
| Disease Keywords | Cancer |
| Model | Tumour line |
| Relevance | Established from a small squamous cell carcinoma (SSC) of the tongue (<20mm in size) of a 32 year-old male patient. STNMP stage 1, well differentiated, node negative tumour. Aneuploid- dual G-/G1 peak on cell cycle analysis by flow cytometry. Mutant p53, codon 244 exon 7; G to T; wild type K-, N- and Ha-ras. Tumourigenic in athymic nude mice, both by subcutaneous injection and injection into the floor of the mouth. By short tandem repeat (STR)-PCR analysis the Y chromosome could not be detected in this cell line when tested at ECACC. It is a known phenomenon that SSC cell lines can lose their Y chromosome. |
| Research Area | Cancer |
| Growth/Phenotype Keywords | Adherent |
| Recommended Growing Conditions | Split sub-confluent cultures (70-80%) 1:8 to 1:10 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Suggested seeding density 5 x 1000 cells/cm². Cells can take approximately 10 minutes to detach, an alternative is to trypsinise 2 to 3 times with fresh trypsin for shorter periods for each trypsin application. Avoid knocking flasks during the trypsinisation process as this can lead to loss of viability. DMEM:HAMS F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 0.5 ug/ml sodium hydrocortisone succinate |
| Cellosaurus ID | CVCL_2457 |
References: 10 entries
Yeudall et al. 1995. Eur J Cancer B Oral Oncol. 31B(2):136-43. PMID: 7633286.
Presence of human papillomavirus sequences in tumour-derived human oral keratinocytes expressing mutant p53.
Europe PMC ID: 7633286
Prime et al. 1994. Int J Cancer. 56(3):406-12. PMID: 7508893.
TGF-beta receptor regulation mediates the response to exogenous ligand but is independent of the degree of cellular differentiation in human oral keratinocytes.
Europe PMC ID: 7508893
Prime et al. 1994. Br J Cancer. 69(1):8-15. PMID: 8286215.
Epidermal growth factor and transforming growth factor alpha characteristics of human oral carcinoma cell lines.
Europe PMC ID: 8286215
Yeudall et al. 1993. Eur J Cancer B Oral Oncol. 29B(1):63-7. PMID: 8180579.
Ras gene point mutation is a rare event in premalignant tissues and malignant cells and tissues from oral mucosal lesions.
Europe PMC ID: 8180579
Prime et al. 1990. J Pathol. 160(3):259-69. PMID: 1692339.
The behaviour of human oral squamous cell carcinoma in cell culture.
Europe PMC ID: 1692339
Add a reference
References: 10 entries
Yeudall et al. 1995. Eur J Cancer B Oral Oncol. 31B(2):136-43. PMID: 7633286.
Presence of human papillomavirus sequences in tumour-derived human oral keratinocytes expressing mutant p53.
Prime et al. 1994. Int J Cancer. 56(3):406-12. PMID: 7508893.
TGF-beta receptor regulation mediates the response to exogenous ligand but is independent of the degree of cellular differentiation in human oral keratinocytes.
Prime et al. 1994. Br J Cancer. 69(1):8-15. PMID: 8286215.
Epidermal growth factor and transforming growth factor alpha characteristics of human oral carcinoma cell lines.
Yeudall et al. 1993. Eur J Cancer B Oral Oncol. 29B(1):63-7. PMID: 8180579.
Ras gene point mutation is a rare event in premalignant tissues and malignant cells and tissues from oral mucosal lesions.
Prime et al. 1990. J Pathol. 160(3):259-69. PMID: 1692339.
The behaviour of human oral squamous cell carcinoma in cell culture.
Add a reference
