THCECb Cell Line
Invented by Prof Michael Apicella from The University Of Iowa
Invented at The University Of Iowa
- Datasheet
- References (1)
- Inventor Info
Info
| Catalogue Number | 153755 |
| Antigen/Gene or Protein Targets | Sexually transmitted diseases (STIs) |
| Host | Human |
| Tissue | Cervix |
| Disease Keywords | Neisseria gonorrhoeae infection, Gonorrhea |
| Model | Immortalised Line |
| Relevance |
This cell line was developed to study Neisseria gonorrhoeae (Gonorrhea) infection in women. Women with N. gonorrhoeae are often asymptomatic leading to chronic infections, upper genital tract infections leading to pelvic inflammatory disease, and increase risk for contracting HIV. This cell line has been used in studying biofilm production of N. gonorrhoeae which can exacerbate the illness and infection. This cell line expresses complement receptor type III on the surface and secretes complement components, necessary for gonococcal attachment and invasion of cervical epithelial cells. |
| Production Details | This cell line was derived from primary cervical cells obtained from cervical biopsies and immortalized with E6/E7. |
| Conditional | No |
| Research Area | Immunology |
| Recommended Growing Conditions | Serum-free keratinocyte growth media (K-SFM) supplement 500 mL of basal media with 12.5 mg BPE and 0.08 ug EGF. Media may be supplemented with 15 PenStrep for routine maintenance. |
