NS0 Cell Line
Invented at Medical Research Council
- Datasheet
- References (9)
- Inventor Info
Info
| Catalogue Number | 153797 |
| Parental Line | NS1 (P3/NS1/1-Ag4-1) |
| Host | Mouse |
| Disease Keywords | Murine myeloma |
| Relevance |
A non-secreting cell line used to generate antibody secreting hybridoma cell lines. Background and Research Application NS0 is a non-Ig secreting, non-light chain-synthesising subclone of NS-1 (P3/NS1/1-Ag4-1). The cells are resistant to 10µM azaguanine, cells die in the presence of HAT medium. NS0 is widely used as a fusion partner to generate antibody secreting hybridoma cell lines and is also used as a model B-cell line. The NS0 cell line is the property of MRC. Ximbio is unable to supply this cell line or derivatives of this cell line to commercial entities unless a license has been first secured from MRC, negotiated by LifeArc (formerly known as MRC Technology); requests for commercial use of this cell line will be forwarded to LifeArc. |
| Research Area | Other |
| Growth/Phenotype Keywords | Suspension |
| Recommended Growing Conditions | Culture medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). |
| Notes |
Points of Interest NS0 Cell Line from murine myeloma has been used to culture NS0 cells expressing CD19scFv (a binding domain of B-lymphocyte antigen CD19) to screen hybridoma clones. Concentration Vial has between 1-5 million cells as standard, however this may vary. |
| Cellosaurus ID | CVCL_3940 |
References: 9 entries
Lau et al. 2020. MAbs. 12(1):1686319. PMID: 31671278.
Low et al. 2020. MAbs. 12(1):1709322. PMID: 31924119.
Challa et al. 2019. MAbs. 11(5):848-860. PMID: 30964743.
Cao et al. 2019. MAbs. 11(3):489-499. PMID: 30786796.
Loebrich et al. 2019. MAbs. 11(2):335-349. PMID: 30252592.
Wada et al. 2019. MAbs. 11(2):350-372. PMID: 30466347.
Sasso et al. 2018. MAbs. 10(5):730-737. PMID: 29658818.
Bradbury et al. 2018. MAbs. 10(4):539-546. PMID: 29485921.
Galfrè et al. 1981. Methods Enzymol. 73(Pt B):3-46. PMID: 7300683.
Add a reference
References: 9 entries
Lau et al. 2020. MAbs. 12(1):1686319. PMID: 31671278.
Low et al. 2020. MAbs. 12(1):1709322. PMID: 31924119.
Challa et al. 2019. MAbs. 11(5):848-860. PMID: 30964743.
Cao et al. 2019. MAbs. 11(3):489-499. PMID: 30786796.
Loebrich et al. 2019. MAbs. 11(2):335-349. PMID: 30252592.
Wada et al. 2019. MAbs. 11(2):350-372. PMID: 30466347.
Sasso et al. 2018. MAbs. 10(5):730-737. PMID: 29658818.
Bradbury et al. 2018. MAbs. 10(4):539-546. PMID: 29485921.
Galfrè et al. 1981. Methods Enzymol. 73(Pt B):3-46. PMID: 7300683.
Add a reference
