pROSA26-Puro-iRFP720 CRISPR vector
Invented by Dr Andrew Stephens from Hudson Institute Of Medical Research , Amy Wilson from Hudson Institute Of Medical Research
Invented at Hudson Institute Of Medical Research
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Info
| Catalogue Number | 153871 |
| Backbone Size (bp) | 8018 |
| Bacterial Resistance | Ampicillin |
| Selectable Markers | Puromycin |
| Vector Type | Mammalian expression, CRISPR, shuttle |
| Antigen/Gene or Protein Targets | Gt(ROSA)26Sor; ROSA26 |
| Positive Control | Contains iRFP720 for positive transfection/integration control |
| Relevance |
CRISPR donor vector targeting ROSA26 locus in mouse cells. Contains left and right ROSA26 homology arms with AsisI/MluI cloning site. Use for genomic integration of any gene of interest into ROSA26 locus. Use in combination with pROSA-GUIDE containing validated gRNA for CAS9 targeting to ROSA26. Species: Mus musculus (house mouse) Tag: Myc, FLAG Cloning method: Restriction Enzyme 5’ cloning site: AsiSI 3’ cloning site: MluI 5’ sequencing primer: CMV Forward |
| Research Area | Gene Expression |
| Notes |
Cancer Research Technology Limited (trading research tools as Ximbio) has been granted a non-exclusive license to the CRISPR-Cas9 technology by ERS Genomics Ltd under the patent rights listed here. This license from ERS Genomics Ltd allows Ximbio to develop and commercialise CRISPR-Cas9 modified cell lines and vectors for research use only. Ximbio can provide these CRISPR-Cas9 research tools to companies under a label-use only license. |
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