pROSA-GUIDE
Invented by Dr Andrew Stephens from Hudson Institute Of Medical Research , Amy Wilson from Hudson Institute Of Medical Research
Invented at Hudson Institute Of Medical Research
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Info
| Catalogue Number | 153872 |
| Backbone Size (bp) | 11530 |
| Bacterial Resistance | Ampicillin |
| Selectable Markers | Puromycin |
| Vector Type | Mammalian Expression, Lentiviral, CRISPR |
| Antigen/Gene or Protein Targets | Gt(ROSA)26Sor; ROSA26 |
| Positive Control | - |
| Research Area | Fluorescent Cell Imaging, Gene Expression |
| Notes |
Validated gRNA targeting ROSA26 locus in mouse cells. Use in combination with pROSA-puro-GFP CRISPR or pROSA-puro-iRFP720 CRISPR to integrate any gene of interest at ROSA26 locus in mouse cells. gRNA in plentiCRISPRv1 backbone. gRNA sequence based on Quadros et al 2015 PMID: 25834785. Species: Mus musculus (house mouse) Cloning method: Restriction Enzyme 5’ cloning site: BsmbI 3’ cloning site: BsmbI 5’ sequencing primer: U6 Forward |
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