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- References (2)
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| Catalogue Number | 153942 |
| Antigen/Gene or Protein Targets | Escherichia coli cells cannot metabolize glucose. |
| Classification | Escherichia coli |
| Bacterial Resistance | Kanamycin, chloramphenicol |
| Relevance | Microbial fermentation processes are used to generate a myriad of comedic chemicals representing market segments valued in the billions of dollars. Microbial metabolic pathways are not though, naturally optimal for the generation of a desired chemical, rather they have evolved for the benefit of the organism. Metabolic engineering is the targeted and rational alteration of metabolism, and it involves the redirection of intra-cellular processes to generate a new product or a product in much higher yields and/or faster rates. UGA researchers have developed a collection of engineered E. coli strains that are capable of (i) producing pyruvate, (ii) used in the co-fermentation – at the same rate - of mixed pentoses and hexoses, or (iii) reducing the formation of acetate by-product of fermentations, under different metabolic conditions. These strains are optimal starting points for the development of novel derivative strains for industrial use |
| Genomic Feature | Hfr zbi::Tn10 poxB1 Δ(aceEF) rpsLpps-4 pfl-1 ldhA::Kan atpFH::Cam |
| Notes | TYA medium, 10g/L tryptone, 5g/L NaCl, 1g/L yeast extract, 1.36 g/L sodium acetate trihydrate Na(CH3COO)?3H2O, pH adjusted to 7.0, aerobic 37C. Provided as lyophilized culture. |
| Research Area | Metabolism, Microbiology, Bacteriology |
References: 2 entries
Zhu et al. 2008. Appl Environ Microbiol. 74(21):6649-55. PMID: 18806005.
High glycolytic flux improves pyruvate production by a metabolically engineered Escherichia coli strain.
Europe PMC ID: 18806005
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References: 2 entries
Zhu et al. 2008. Appl Environ Microbiol. 74(21):6649-55. PMID: 18806005.
High glycolytic flux improves pyruvate production by a metabolically engineered Escherichia coli strain.
Add a reference
Inventor Information
Inventors
|
Mark Eiteman |
