Immortalised Human Fibroblast Cell Line
Invented by Professor. Pranab K
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Info
| Catalogue Number | 154112 |
| Parental Line | Human foreskin |
| Host | Human |
| Tissue | Fibroblast |
| Model | Immortalised Line |
| Relevance | Immortalised human fibroblasts are widely employed as a model system for cell and molecular biology studies |
| Production Details | Fibroblast cultures were establised from neonatal foreskin using standard methods. Fibroblasts were transfected with HPV16 genes E6 and E7 using the retroviral construct LXCN16E6E7. The E6E7 genes are under the control of the MMLV promoter-enhancer sequence. In additon the vector contained a geneticin resistance gene. The retroviral particles were produced by the packaging cell line PA317. The critical concentration of geneticin for transformed selection was 1mg/ml. |
| Research Area | Cell Type or Organelle Marker |
| Growth/Phenotype Keywords | Cells were routinely passaged 1:4 at confluency |
| Recommended Growing Conditions | DMEM:M199 (1:4) supplemented with 10% FBS |
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