MDA-MB-231 TetOn-3G ROCK1 cell line
Invented by Heather Mckinnon , Justin Bower , Daniel Croft
Invented at Cancer Research UK Glasgow: The Beatson Institute
- Datasheet
- References (2)
- Inventor Info
Info
| Catalogue Number | 154150 |
| Antigen/Gene or Protein Targets | ROCK1 |
| Parental Line | MDA-MB-231 D3H2LN-Luc cell line |
| Synonyms | Rho-associated protein kinase 1, Renal carcinoma antigen NY-REN-35, Rho-associated, coiled-coil-containing protein kinase 1, Rho-associated, coiled-coil-containing protein kinase I, ROCK-I, p160 ROCK-1, p160ROCK |
| Host | Human |
| Tissue | Breast |
| Disease Keywords | Triple negative breast cancer (TNBC) |
| Model | Transgenic |
| Relevance |
This cell line has been engineered to enable tetracycline inducible expression of the kinase ROCK1. ROCK1 is a serine/threonine kinase which acts as a key regulator of the actin-myosin cytoskeleton and cell polarity. ROCK1 promotes contractile force generation and therefore is a modulator of cell motility and tumour cell invasion. The cell line is a derivative of a human breast cancer cell line shown to reliably metastasize to clinically relevant sites (the lungs and lymph nodes) when implanted orthotopically in mice (i.e. when implanted in the breast pad of mice). The cell line expresses luciferase enabling detection of the location of the cells using bioluminescent imaging. Useful for the study of breast cancer cell cytoskeleton reorganisation and cell migration. This cell line is derived from a triple negative breast cancer (TNBC) meaning it does not express oestrogen, progesterone or HER2 receptors. |
| Conditional | Yes |
| Conditional Description | MDA-MB-231 D3H2LN-Luc cell line that expresses tetracycline (Tet)-regulated transactivator Tet-On 3G regulated expression of ROCK1. Therefore ROCK1 expression is induced in the presence of doxycycline. |
| Research Area | Cancer, Cell Structure and Motility, Drug Discovery & Development, Genetic Studies Tools |
| Growth/Phenotype Keywords | Adherent cell line. Membrane blebbing/cytoskeleton reorganisation and migration induced in the presence of doxycycline. |
| Recommended Growing Conditions | 10% FBS/MEM-EBSS/NEAA/NaPyr/Glutamine |
| Notes | Tet-On System owned by TET Systems GmbH & Co. KG. |
References: 2 entries
Unbekandt et al. 2014. Cell Commun Signal. 12:54. PMID: 25288205.
A novel small-molecule MRCK inhibitor blocks cancer cell invasion.
Europe PMC ID: 25288205
Add a reference
References: 2 entries
Unbekandt et al. 2014. Cell Commun Signal. 12:54. PMID: 25288205.
A novel small-molecule MRCK inhibitor blocks cancer cell invasion.
Add a reference
Inventor Information
Inventors
|
Heather Mckinnon |
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|
Justin Bower |
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Daniel Croft |
